Characterization of Staphylococcus aureus from Humans and a Comparison with İsolates of Animal Origin, in North Dakota, United States
| Autor: | Catherine M. Logue, Ryan M Stepan, Julie S. Sherwood, Esra Büyükcangaz, Ryan J. Koslofsky, Valeria Velasco |
|---|---|
| Přispěvatelé: | Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı., Büyükcangaz, Esra, AAL-2323-2020 |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Bacterium identification
Identification Science & technology - other topics Antibiotic resistance lcsh:Medicine Strains Bacterial strain Molecular typing Multidrug resistance medicine.disease_cause Procedures MecA gene Bacterial protein Staphylococcus infection Ciprofloxacin Bacterial transmission Drug Resistance Multiple Bacterial RNA Ribosomal 16S Prevalence Cluster Analysis lcsh:Science Genetic similarity Phylogeny Multidisciplinary Bacterial gene Mrsa Bovine Microbial sensitivity test Staphylococcal Infections Methicillin-resistant Classification Nose cavity Chicken Erythromycin Normal human Staphylococcus aureus North Dakota Penicillin derivative Animals Meticillin Cefoxitin Food control Nasal Cavity Human Research Article Methicillin-Resistant Staphylococcus aureus Bacterium isolate Meat RNA 16S Clone Microbial Sensitivity Tests Biology Staphylococcal infections Infections Microbiology Multidisciplinary sciences Article Young Adult Bacterial Proteins medicine Genetics Humans Raw meat Sheep Animal SCCmec lcsh:R Tetracycline biochemical phenomena metabolism and nutrition medicine.disease Nonhuman bacterial infections and mycoses Methicillin-resistant Staphylococcus aureus United States Multiple drug resistance Drug effect PVL gene Isolation and purification Food Microbiology Multilocus sequence typing Strain difference lcsh:Q Genetic variability Cattle Comparative study Controlled study Chickens Methicillin resistant Staphylococcus aureus Multilocus Sequence Typing |
| Zdroj: | PLoS ONE PLoS ONE, Vol 10, Iss 10, p e0140497 (2015) |
| ISSN: | 1932-6203 |
| Popis: | Different clones of methicillin-susceptible (MSSA) and methicillin-resistant (MRSA) Staphylococcus aureus have been found in humans as well as in animals and retail meat. However, more information about the genetic characteristics and similarities between strains is needed. The aim of this study was to identify and characterize Staphylococcus aureus from humans, and to compare their characteristics with isolates of animal origin. A total of 550 nasal swabs were taken from healthy humans, and S. aureus was isolated and identified. Positive S. aureus isolates were subjected to molecular typing and susceptibility testing. In addition, 108 MRSA isolates recovered from clinical patients in the state of North Dakota and 133 S. aureus isolates from animals and meat previously analyzed were included. The nasal carriage of S. aureus in healthy people was 7.6% and, in general, clones were genetically diverse. None of the S. aureus strains obtained from healthy people were mecA-or PVL-positive. A total of 105 (97.2%) MRSA isolates from clinical cases harbored the mecA gene and 11 (10.2%) isolated from blood stream infections harbored the PVL gene. The most common resistance profile among S. aureus from healthy people was penicillin, and from clinical cases were erythromycin-penicillin-ciprofloxacin. The rate of multidrug resistance (MDR) was 70% in humans. Most of the S. aureus harboring mecA and PVL genes were identified as ST5 and ST8, and exhibited MDR. However, S. aureus isolates of animal origin used for comparison exhibited a lower rate of MDR. The most common resistance profiles in isolates of animal origin were penicillin-tetracycline and penicillin-tetracycline-erythromycin, in animals and raw meat, respectively. The ST5 was also found in animals and meat, with ST9 and ST398 being the major clones. The genetic similarity between clones from humans and meat suggests the risk of spread of S. aureus in the food chain. Dean's Office, College of Veterinary Medicine, Iowa State University, Ames, Iowa Dean's Office, College of Agriculture, Food Systems and Natural Resources College, North Dakota State University |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|