Different endothelins stimulate cytokine production by peritoneal macrophages and microglial cell line
| Autor: | Marina Saresella, Donatella Taramelli, Livianna Speciale, K. Roda, Pasquale Ferrante |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
medicine.hormone
Necrosis medicine.medical_treatment Immunology Cell Culture Techniques Stimulation Mice Inbred Strains Proinflammatory cytokine Nitric oxide Endothelins chemistry.chemical_compound Interferon-gamma Mice medicine Immunology and Allergy Animals Autocrine signalling Endothelin-2 business.industry Interleukin-6 Interleukins Cell biology Cytokine chemistry Macrophages Peritoneal Female Microglial cell Microglia medicine.symptom business Interleukin-1 Research Article |
| Zdroj: | ResearcherID |
| ISSN: | 0019-2805 |
| Popis: | Endothelins (ETs), potent vasoconstricting peptides, are produced by macrophages upon stimulation and may participate in the amplification or regulation of the inflammatory response. However, it is not clear whether ETs can act in an autocrine manner on macrophages and which role they play in relationship with other cytokines. To address these issues, we studied the effects of ETs on the production of inflammatory cytokines by mouse peritoneal macrophages or by a retrovirus-transformed microglial cell line. Here, we report that ET-2, but not ET-1 or ET-3, is able to stimulate the production of interleukin-1 (IL-1) and interleukin-6 (IL-6) by peptone-elicited mouse macrophages (pMO). In contrast, ET-3 and ET-1, but not ET-2, are active on microglial cells. No tumour necrosis factor-alpha (TNF-alpha) or nitric oxide (NO) were detected in the supernatants of ET-stimulated cultures. The activity of ET-2 on pMO was time and dose dependent and was inhibited by the addition of ETA and ETB receptor antagonists, BQ123 and IRL1038, respectively. In addition, when pMO were stimulated by interferon-gamma (IFN-gamma) in the presence of ET-2, a significant inhibition of IL-6 and IL-1 production was observed compared with the effects of the same doses of IFN-gamma or ET-2 used separately. The inhibition was specifically due to the activity of ET-2, since it was reversed by the addition of BQ123 or IRL1038. Similar results were seen when the content of NO in the supernatants of pMO stimulated by IFN-gamma plus ET-2 was evaluated. These results suggest that ETs may possess both a pro-inflammatory action on macrophages from different tissues and a regulatory activity on IFN-gamma. |
| Databáze: | OpenAIRE |
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