Histological age estimation of the eggs ofCalliphora vicinaRobineau Desvoidy (Diptera: Calliphoridae)
| Autor: | Melanie S. Archer, Michael Pais |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Veterinary medicine
Calliphora vicina forensic entomology Blow fly 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) Pathology and Forensic Medicine Analytical Chemistry histology 03 medical and health sciences Calliphoridae 0302 clinical medicine eggs embryology 030216 legal & forensic medicine Forensic entomology lcsh:K5000-5582 Physical and Theoretical Chemistry Fixation (histology) biology lcsh:Public aspects of medicine 010401 analytical chemistry lcsh:RA1-1270 Histology biology.organism_classification 0104 chemical sciences Staining Psychiatry and Mental health Age estimation Anthropology lcsh:Criminal law and procedure Forensic science |
| Zdroj: | Forensic Sciences Research, Vol 3, Iss 1, Pp 40-51 (2018) |
| ISSN: | 2471-1411 2096-1790 |
| Popis: | Aging blow fly eggs can be critical to a forensic investigation, but there are currently no forensically useful timelines describing internal anatomical changes in embryological development. This is partly due to the lack of an economical, rapid and technically simple histological technique to allow mass production of slides for research and casework. We present a histological method that uses a slightly modified standard laboratory processing run with 1 h fixation in 10% formalin, 2 h softening in Molliflex and Haemotoxylin and Eosin (H&E) staining. We also present a summary of the internal anatomical changes that can be visualized using our technique in the developing eggs of Calliphora vicina Robineau-Desvoidy (Diptera: Calliphoridae). We examined eggs from at least three different females grown at 15 °C and sampled at 6 h intervals, and eggs grown at 20 °C and sampled at 3 h intervals. Blind aging trials demonstrated that it is possible to accurately age material grown at 20 °C to within 6 h (but attempts to further narrow this interval resulted in errors in one-third of cases). It was also possible to see sufficient anatomical detail to age eggs preserved for forensic casework 5, 8, 9, 10 and 11 years previously. Additionally, we determined that section quality was improved by 5 s fixation in hot water prior to preservation in ethanol. However, hot water fixation for longer than this increased the level of section artefact. |
| Databáze: | OpenAIRE |
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