Increased complexity of t(11;14) rearrangements in plasma cell neoplasms compared with mantle cell lymphoma

Autor: Patricia T. Greipp, Jess F. Peterson, Kathryn E. Pearce, Joanna C. Dalland, Shaji Kumar, Rhett P. Ketterling, Rebecca L. King, Linda B. Baughn, Neeraj Sharma, Reid G. Meyer, James B. Smadbeck
Rok vydání: 2021
Předmět:
Cancer Research
mantle cell lymphoma
next‐generation sequencing
Lymphoma
Mantle-Cell
Biology
Translocation
Genetic
Cyclin D1 Overexpression
CCND1
03 medical and health sciences
0302 clinical medicine
Cyclin D1
t(11
14)(q13
q32)
immune system diseases
hemic and lymphatic diseases
Cytology
Genetics
medicine
Humans
fluorescence in situ hybridization
Neoplasms
Plasma Cell
neoplasms
Research Articles
In Situ Hybridization
Fluorescence
Chromosomes
Human
Pair 14
Gene Rearrangement
medicine.diagnostic_test
IGH
Chromosomes
Human
Pair 11
Plasma cell neoplasm
medicine.disease
Molecular biology
Paraffin embedded tissue
medicine.anatomical_structure
plasma cell neoplasms
030220 oncology & carcinogenesis
mate‐pair sequencing
Mantle cell lymphoma
Bone marrow
Research Article
Fluorescence in situ hybridization
Zdroj: Genes, Chromosomes & Cancer
ISSN: 1098-2264
1045-2257
Popis: Plasma cell neoplasms (PCN) and mantle cell lymphoma (MCL) can both harbor t(11;14)(q13;q32) (CCND1/IGH), usually resulting in cyclin D1 overexpression. In some cases, particularly at low levels of disease, it can be morphologically challenging to distinguish between these entities in the bone marrow (BM) since PCN with t(11;14) are often CD20-positive with lymphoplasmacytic cytology, while MCL can rarely have plasmacytic differentiation. We compared the difference in CCND1/IGH by fluorescence in situ hybridization (FISH) in PCN and MCL to evaluate for possible differentiating characteristics. We identified 326 cases of MCL with t(11;14) and 279 cases of PCN with t(11;14) from either formalin-fixed, paraffin embedded tissue or fresh BM specimens. The "typical", balanced CCND1/IGH FISH signal pattern was defined as 3 total CCND1 signals, 3 total IGH signals, and 2 total fusion signals. Any deviation from the "typical" pattern was defined as an "atypical" pattern, which was further stratified into "gain of fusion" vs. "complex" patterns. There was a significantly higher proportion of cases that showed an atypical FISH pattern in PCN compared to MCL (53% versus 27%, P
Databáze: OpenAIRE
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