Increased complexity of t(11;14) rearrangements in plasma cell neoplasms compared with mantle cell lymphoma
Autor: | Patricia T. Greipp, Jess F. Peterson, Kathryn E. Pearce, Joanna C. Dalland, Shaji Kumar, Rhett P. Ketterling, Rebecca L. King, Linda B. Baughn, Neeraj Sharma, Reid G. Meyer, James B. Smadbeck |
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Rok vydání: | 2021 |
Předmět: |
Cancer Research
mantle cell lymphoma next‐generation sequencing Lymphoma Mantle-Cell Biology Translocation Genetic Cyclin D1 Overexpression CCND1 03 medical and health sciences 0302 clinical medicine Cyclin D1 t(11 14)(q13 q32) immune system diseases hemic and lymphatic diseases Cytology Genetics medicine Humans fluorescence in situ hybridization Neoplasms Plasma Cell neoplasms Research Articles In Situ Hybridization Fluorescence Chromosomes Human Pair 14 Gene Rearrangement medicine.diagnostic_test IGH Chromosomes Human Pair 11 Plasma cell neoplasm medicine.disease Molecular biology Paraffin embedded tissue medicine.anatomical_structure plasma cell neoplasms 030220 oncology & carcinogenesis mate‐pair sequencing Mantle cell lymphoma Bone marrow Research Article Fluorescence in situ hybridization |
Zdroj: | Genes, Chromosomes & Cancer |
ISSN: | 1098-2264 1045-2257 |
Popis: | Plasma cell neoplasms (PCN) and mantle cell lymphoma (MCL) can both harbor t(11;14)(q13;q32) (CCND1/IGH), usually resulting in cyclin D1 overexpression. In some cases, particularly at low levels of disease, it can be morphologically challenging to distinguish between these entities in the bone marrow (BM) since PCN with t(11;14) are often CD20-positive with lymphoplasmacytic cytology, while MCL can rarely have plasmacytic differentiation. We compared the difference in CCND1/IGH by fluorescence in situ hybridization (FISH) in PCN and MCL to evaluate for possible differentiating characteristics. We identified 326 cases of MCL with t(11;14) and 279 cases of PCN with t(11;14) from either formalin-fixed, paraffin embedded tissue or fresh BM specimens. The "typical", balanced CCND1/IGH FISH signal pattern was defined as 3 total CCND1 signals, 3 total IGH signals, and 2 total fusion signals. Any deviation from the "typical" pattern was defined as an "atypical" pattern, which was further stratified into "gain of fusion" vs. "complex" patterns. There was a significantly higher proportion of cases that showed an atypical FISH pattern in PCN compared to MCL (53% versus 27%, P |
Databáze: | OpenAIRE |
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