A rat liver system that catalyses a pyridoxal phosphate-independent αβ-elimination
| Autor: | J. A. Fowler, J. H. Thomas, N. Tudball |
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| Rok vydání: | 1969 |
| Předmět: |
History
Tritium Cofactor Education Serine chemistry.chemical_compound Sulfur Isotopes Isoniazid medicine Animals Pyridoxal phosphate Pyridoxal chemistry.chemical_classification biology Sulfates Alanine Transaminase Articles Metabolism Pyridoxine Enzymes Rats Computer Science Applications Enzyme Liver Biochemistry Alanine transaminase chemistry Pyridoxal Phosphate biology.protein Vitamin B 6 Deficiency Protein Binding medicine.drug |
| Zdroj: | Biochemical Journal. 114:299-305 |
| ISSN: | 0306-3283 |
| Popis: | 1. Attempts were made to demonstrate the presence of pyridoxal phosphate in the rat liver system catalysing the alphabeta-elimination of l-serine O-sulphate. 2. Methods designed to resolve protein-bound cofactor, spectroscopic examination of a purified enzyme system and attempted reactivation of apo-(alanine aminotransferase) failed to demonstrate the presence of pyridoxal phosphate. 3. The activity of the alphabeta-eliminating system remained constant in vitamin B(6)-deficient animals even though the activities of other pyridoxal phosphate-dependent systems fell markedly. 4. The metabolism of l-serine O[(35)S]-sulphate in vivo appears to be normal in vitamin B(6)-deficient animals. 5. No incorporation of tritium into the alphabeta-eliminating system occurred after administration of tritiated pyridoxine to experimental animals. |
| Databáze: | OpenAIRE |
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