Enhanced hypovirus transmission by engineered super donor strains of the chestnut blight fungus, Cryphonectria parasitica, into a natural population of strains exhibiting diverse vegetative compatibility genotypes
| Autor: | Matthew T. Kasson, Donald L. Nuss, Amy M. Metheny, Cameron M. Stauder, M. L. Double, Dong-Xiu Zhang, W. L. MacDonald |
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| Rok vydání: | 2019 |
| Předmět: |
Genotype
Population Virulence Biology Fagaceae 03 medical and health sciences Ascomycota Virology Chestnut blight RNA Viruses Hypovirus Cryphonectria education Plant Diseases 030304 developmental biology Genetics 0303 health sciences education.field_of_study fungi 030302 biochemistry & molecular biology biology.organism_classification Biological Control Agents Natural population growth Horizontal transmission |
| Zdroj: | Virology. 528:1-6 |
| ISSN: | 0042-6822 |
| Popis: | Horizontal transmission of virulence attenuating hypoviruses of Cryphonectria parasitica is restricted by an allorecognition system termed vegetative incompatibility (vic). A super donor formulation of two engineered C. parasitica strains (SD328/SD82) with gene disruptions at four of six vic loci transmitted hypovirus to strains in the laboratory independent of vic genotype. We now report the transmission of hypovirus by the SD328/82 formulation to a diverse, natural C. parasitica population infecting American chestnut in a forest setting. Hypovirulent (HV) isolates were recovered from 94% of cankers treated with the hypovirus-infected SD328/82 formulation compared to 51% of cankers treated with a hypovirus-infected EU5/6 formulation (strains having the same vic genotypes as SD strains but lacking vic gene disruptions). Overall, the SD328/82 formulation transmitted hypovirus into more divergent vic genotypes compared to the EU5/6 formulation. These results demonstrate the SD328/82 formulation can serve as an enhanced hypovirus vector for highly divergent C. parasitica populations. |
| Databáze: | OpenAIRE |
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