Frequent Assembly of Chimeric Complexes in the Protein Interaction Network of an Interspecies Yeast Hybrid
| Autor: | Christian R. Landry, Rohan Dandage, Kyung-Mee Moon, Richard Greg Stacey, Caroline M. Berger, Leonard J. Foster, Isabelle Gagnon-Arsenault |
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| Rok vydání: | 2020 |
| Předmět: |
Proteomics
Saccharomyces cerevisiae Biology AcademicSubjects/SCI01180 Saccharomyces uvarum Protein–protein interaction 03 medical and health sciences 0302 clinical medicine Interaction network Genetics Protein Interaction Maps Molecular Biology hybridization Ecology Evolution Behavior and Systematics Discoveries 030304 developmental biology 0303 health sciences AcademicSubjects/SCI01130 Prefoldin complex biology.organism_classification Phenotype Fusion protein Yeast Proteostasis protein–protein interaction Proteome Hybridization Genetic 030217 neurology & neurosurgery |
| Zdroj: | Molecular Biology and Evolution |
| ISSN: | 1537-1719 |
| Popis: | Hybrids between species often show extreme phenotypes, including some that take place at the molecular level. In this study, we investigated the phenotypes of an interspecies diploid hybrid in terms of protein-protein interactions inferred from protein correlation profiling. We used two yeast species, Saccharomyces cerevisiae and Saccharomyces uvarum, which are interfertile, but yet have proteins diverged enough to be differentiated using mass spectrometry. Most of the protein-protein interactions are similar between hybrid and parents, and are consistent with the assembly of chimeric complexes, which we validated using an orthogonal approach for prefoldin complex. We also identify instances of altered protein-protein interactions in the hybrid, for instance in complexes related to proteostasis and in mitochondrial protein complexes. Overall, this study uncovers likely frequent occurrence of chimeric protein complexes with few exceptions, which may result from incompatibilities or imbalances between the parental proteins. |
| Databáze: | OpenAIRE |
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