Improved pregnancy rate after transfer of embryos grown in human fallopian tubal cell coculture
| Autor: | Shan S. Ratnam, Soon-Chye Ng, Barry Marshall, C. Anandakumar, Chui-Yee Fong, Rohini Edirisinghe, Ariff Bongso |
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| Rok vydání: | 1992 |
| Předmět: |
Adult
medicine.medical_specialty medicine.medical_treatment Fertilization in Vitro Biology Epithelium Embryonic and Fetal Development Pregnancy Culture Techniques medicine Humans Prospective Studies Blastocyst Fallopian Tubes Gynecology Assisted reproductive technology In vitro fertilisation Pronucleus Obstetrics and Gynecology Embryo Transfer medicine.disease Embryo transfer Pregnancy rate medicine.anatomical_structure Reproductive Medicine embryonic structures Gestation Female |
| Zdroj: | Fertility and Sterility. 58:569-574 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/s0015-0282(16)55265-0 |
| Popis: | Objective To evaluate the embryonic behavior in vitro and the pregnancy and implantation rates of embryos grown in a human ampullary cell coculture system. Design In a prospective study, two pronuclei embryos were cultured on human ampullary feeder layers up to the two to six-cell and blastocyst stages and replaced either as tubal, uterine, or sequential transfers. Setting Assisted reproductive technology program in a university-based hospital. Patients Fifty women with a mean age of 35.6 years who went through a single coculture cycle. Thirty of the patients were admitted for in vitro fertilization (IVF) and 20 for tubal embryo transfer (TET). Results The overall clinical pregnancy rate (PR) for all 50 patients was 44% per cycle (IVF, 37%; TET, 55%) and the implantation rate was 31.8% (IVF, 31.0%; TET, 32.6%). Sixty-eight percent of pregnant patients were over 35years, and 68% had two previously failed assisted reproduction cycles. Five of 9 patients who received sequential transfers became pregnant. Three of the 22 pregnancies aborted (2 after sequential transfer), and there was one ectopic. Overall, 88% of two to six-cell stage embryos were of good quality. Conclusions The human ampullary coculture system produces better quality embryos, increased numbers of blastocysts with improved PRs and implantation rates. The beneficial effects of the feeder layer may be through the release of embryotrophic factors and detoxification of the medium by the cells. Coculture is a new concept in assisted reproduction and has tremendous potential in boosting conception rates by mimicking the in vivo environment. |
| Databáze: | OpenAIRE |
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