The Transcriptomic Landscape of Prostate Cancer Development and Progression: An Integrative Analysis

Autor: Claude Chelala, Stefano Pirrò, Daniel M. Berney, Emanuela Gadaleta, Sakunthala C. Kudahetti, Yanan Zhu, Guoping Ren, Yong-Jie Lu, Xueying Mao, Bernard V. North, Jun Wang, Jacek Marzec, Luis Beltran, Elzbieta Stankiewicz, Amar Ahmad, Solene-Florence Kammerer-Jacquet, Helen Ross-Adams
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Cancers
Cancers, Vol 13, Iss 345, p 345 (2021)
Volume 13
Issue 2
ISSN: 2072-6694
Popis: Simple Summary There is a tremendous amount of gene expression information available for prostate cancer, but very few tools exist to combine the disparate datasets generated across sample types and technical platforms. We present a method of integrating different types of expression data from different study cohorts to increase analytic power, and improve our understanding of the molecular changes underlying the development and progression of prostate cancer from normal to advanced disease. Using this approach, we identified nine additional disease stage-specific candidate genes with prognostic significance, which were not identified in any one study alone. We have developed a free online tool summarizing our results, and making the complete combined dataset available for further translational research. Abstract Next-generation sequencing of primary tumors is now standard for transcriptomic studies, but microarray-based data still constitute the majority of available information on other clinically valuable samples, including archive material. Using prostate cancer (PC) as a model, we developed a robust analytical framework to integrate data across different technical platforms and disease subtypes to connect distinct disease stages and reveal potentially relevant genes not identifiable from single studies alone. We reconstructed the molecular profile of PC to yield the first comprehensive insight into its development, by tracking changes in mRNA levels from normal prostate to high-grade prostatic intraepithelial neoplasia, and metastatic disease. A total of nine previously unreported stage-specific candidate genes with prognostic significance were also found. Here, we integrate gene expression data from disparate sample types, disease stages and technical platforms into one coherent whole, to give a global view of the expression changes associated with the development and progression of PC from normal tissue through to metastatic disease. Summary and individual data are available online at the Prostate Integrative Expression Database (PIXdb), a user-friendly interface designed for clinicians and laboratory researchers to facilitate translational research.
Databáze: OpenAIRE
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