CysK Plays a Role in Biofilm Formation and Colonization by Vibrio fischeri
| Autor: | Priyanka Abhishek Singh, John F. Brooks, Mark J. Mandel, Karen L. Visick, Valerie A. Ray |
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| Rok vydání: | 2015 |
| Předmět: |
Euprymna scolopes
Mutant Genetics and Molecular Biology Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Bacterial Proteins Animals Cysteine Genetic Testing Gene Cysteine metabolism Cysteine Synthase Ecology biology Genetic Complementation Test Decapodiformes Biofilm biochemical phenomena metabolism and nutrition biology.organism_classification Aliivibrio fischeri Vibrio Biosynthetic Pathways Culture Media chemistry Biofilms Mutation Bacteria Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 81:5223-5234 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.00157-15 |
| Popis: | A biofilm, or a matrix-embedded community of cells, promotes the ability of the bacterium Vibrio fischeri to colonize its symbiotic host, the Hawaiian squid Euprymna scolopes . Biofilm formation and colonization depend on syp , an 18-gene polysaccharide locus. To identify other genes necessary for biofilm formation, we screened for mutants that failed to form wrinkled colonies, a type of biofilm. We obtained several with defects in genes required for cysteine metabolism, including cysH , cysJ , cysK , and cysN . The cysK mutant exhibited the most severe wrinkling defect. It could be complemented with a wild-type copy of the cysK gene, which encodes O -acetylserine sulfhydrolase, or by supplementing the medium with additional cysteine. None of a number of other mutants defective for biosynthetic genes negatively impacted wrinkled colony formation, suggesting a specific role for CysK. CysK did not appear to control activation of Syp regulators or transcription of the syp locus, but it did influence production of the Syp polysaccharide. Under biofilm-inducing conditions, the cysK mutant retained the same ability as that of the parent strain to adhere to the agar surface. The cysK mutant also exhibited a defect in pellicle production that could be complemented by the cysK gene but not by cysteine, suggesting that, under these conditions, CysK is important for more than the production of cysteine. Finally, our data reveal a role for cysK in symbiotic colonization by V. fischeri . Although many questions remain, this work provides insights into additional factors required for biofilm formation and colonization by V. fischeri . |
| Databáze: | OpenAIRE |
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