Phosphorylated-Akt overexpression is associated with a higher risk of brain metastasis in patients with non-small cell lung cancer
| Autor: | Bo Liu, Minxiao Yi, Qianxia Li, Hu Han, Yu Jin, Ye Yuan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Biophysics AKT1 Single-nucleotide polymorphism Biochemistry lcsh:Biochemistry 03 medical and health sciences 0302 clinical medicine Non-small cell lung cancer Genotype medicine lcsh:QD415-436 NSCLC Non-small cell lung cancer Lung cancer lcsh:QH301-705.5 Protein kinase B Predictive marker business.industry p-Akt Akt phosphorylation Akt Brain metastasis BM Brain metastases Predict biomarker SNPs Single nucleotide polymorphisms KPS Karnofsky performance status medicine.disease PCI Prophylactic cranial irradiation Single nucleotide polymorphism 030104 developmental biology lcsh:Biology (General) CDH2 N-cadherin 030220 oncology & carcinogenesis Cancer research Immunohistochemistry business Research Article |
| Zdroj: | Biochemistry and Biophysics Reports Biochemistry and Biophysics Reports, Vol 18, Iss, Pp-(2019) |
| ISSN: | 2405-5808 |
| Popis: | Brain metastasis (BM) of non-small cell lung cancer (NSCLC) is relatively common and has a poor prognosis. Moreover, identifying which patients are more likely to develop BM is challenging. Akt, a serine/threonine-specific protein kinase, can be activated in various tumors, including lung cancer, and may be associated with poor prognosis. Here, we used immunohistochemistry to evaluate phosphorylated-Akt (p-Akt) expression in tumor tissues of 99 NSCLC patients. We also analyzed the genotype of the patients for two single nucleotide polymorphisms (SNPs) of the AKT1 gene, rs2498804 and rs2494732. We found that p-Akt expression differs between NSCLC patients and correlates with the risk of BM. Indeed, patients exhibiting medium to high p-Akt expression had a higher incidence of BM than those exhibiting low to no p-Akt expression (39% vs 16%). Our data also show that patients with the rs2498804 GT/GG and rs2494732 CT/TT variant genotypes were more likely to exhibit higher levels of p-Akt expression than those with the rs2498804 TT and rs2494732 CC variant genotypes (35% vs. 24% and 37% vs. 25%, respectively). Our results suggest that the level of expression of p-Akt, which may be affected by the AKT1 genotype, is correlated with the risk of BM. However, further studies are needed to establish p-Akt as a predictive marker for BM in NSCLC patients. Highlights •The level of expression of p-Akt differs significantly between NSCLC patients. • Patients with high p-Akt expression have a higher incidence of brain metastasis. • AKT1 SNP variant genotypes are associated with higher expression levels of p-Akt. • p-Akt is a prospective marker to predict brain metastasis in NSCLC patients. |
| Databáze: | OpenAIRE |
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