Novel Temperature Activation Cell-Based Assay on Thermo-TRP Ion Channels
| Autor: | Michael Dabrowski, Eduardo Aneiros |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Cells
TRPM Cation Channels TRPV Cation Channels Nerve Tissue Proteins Biology Biochemistry Calcium in biology Cell Line Analytical Chemistry Transient Receptor Potential Channels TaqMan Animals Humans Primary cell TRPA1 Cation Channel Ion channel Drug discovery Temperature Molecular biology Fluorescence Rats Cell culture Biophysics Molecular Medicine Biological Assay Calcium Channels Heterologous expression Ion Channel Gating Biotechnology |
| Zdroj: | SLAS Discovery. 14:662-667 |
| ISSN: | 2472-5552 |
| DOI: | 10.1177/1087057109336595 |
| Popis: | The precise temperature control of the ABI Prism 7900HT Sequence Detection System designed for detection of fluores- cence of a biological sample in real-time PCR assays (TaqMan assays) was used to activate Thermo-TRP ion channels, enabling a novel 384-/96-well plate-based assay. Functional pharmacology was verified against the temperature activation using intracellular calcium fluorescence as a measure of ion channel activity. The assay is applicable to both heterologous expression systems and dorsal root ganglia primary cells. This will benefit several analgesic drug discovery programs search- ing for new Thermo-TRP modulators. (Journal of Biomolecular Screening 2009:662-667) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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