Effects of recombinant human granulocyte colony-stimulating factor on central and peripheral T lymphocyte reconstitution after sublethal irradiation in mice
| Autor: | Wan-Jun Sun, Xue-Dong Sun, Hai-lan Hu, Mei Guo, Li Wei, Hong-Xia Zhao, Hui-Sheng Ai |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Cell Survival
Health Toxicology and Mutagenesis T cell T-Lymphocytes Radiation-Protective Agents Biology Radiation Dosage Peripheral blood mononuclear cell Radiation Tolerance Lethal Dose 50 Mice Immune system recombinant human granulocyte colony-stimulating factor Lymphopenia Granulocyte Colony-Stimulating Factor medicine Animals Radiology Nuclear Medicine and imaging Radiation Injuries Cells Cultured Cell Proliferation Mice Inbred BALB C Radiation Dose-Response Relationship Radiation T lymphocyte immune reconstitution Total body irradiation Molecular biology Recombinant Proteins recent thymic emigrants Haematopoiesis Thymocyte medicine.anatomical_structure Treatment Outcome Immunology Irradiation Female CD8 Whole-Body Irradiation |
| Zdroj: | Journal of Radiation Research |
| ISSN: | 1349-9157 0449-3060 |
| Popis: | Granulocyte colony-stimulating factor (G-CSF) is one of the most critical cytokines used for the treatment of acute radiation syndrome (ARS). In addition to the hematopoietic effects of G-CSF on the differentiation and proliferation of myeloid progenitor cells, G-CSF is also known to have immunomodulatory effects. The aim of the present study was to investigate whether G-CSF could accelerate central and peripheral T lymphocyte recovery after a sublethal dose of irradiation. Female BALB/c mice were subjected to 6 Gy of total body irradiation and then were treated with either 100 μg/kg G-CSF or an equal volume of PBS once daily for 14 days. Percentages of thymocyte subpopulations including CD4 - CD8 - , CD4 + CD8 + , CD4 + CD8- and CD4 - CD8+ T cells, peripheral CD3 + , CD4+ and CD8+ cells were analyzed by flow cytometry. Recent thymic emigrants (RTEs) were assessed by real-time polymerase chain reaction (PCR) using primers specific to the 257-bp T cell receptor rearrangement excision circles (sjTRECs). The proliferative capacity of splenic mononuclear cells upon exposure to ConA was measured by using the Cell Count Kit-8 (CCK-8). G-CSF treatment promoted thymocyte regeneration, accelerated the recovery of CD4 + CD8+ cells and increased the frequency of thymocyte sjTRECs. These effects were more prominent at early time points (Day 28) after irradiation. G-CSF also increased the rate of recovery of peripheral CD3 + , CD4+ and CD8+ cells and shortened the period of severe lymphopenia following irradiation. G-CSF also increased the splenic mononuclear cell mitotic responsiveness to ConA more than control-treated cells. Our results show that G-CSF accelerates T cell recovery through both thymic-dependent and thymic-independent pathways, which could be used to increase the rate of immune reconstitution after sublethal irradiation. |
| Databáze: | OpenAIRE |
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