Raw starch fermentation to ethanol by an industrial distiller’s yeast strain of Saccharomyces cerevisiae expressing glucoamylase and α-amylase genes
| Autor: | Il-Chul Kim, Young-Kum Im, Hwanghee Blaise Lee, Ha-Ram Kim, Jong-Eon Chin, Suk Bai, Hyun-Mi Ko |
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| Rok vydání: | 2011 |
| Předmět: |
Debaryomyces
Starch Bioengineering Saccharomyces cerevisiae Ethanol fermentation Applied Microbiology and Biotechnology Fungal Proteins Industrial Microbiology chemistry.chemical_compound Amylase Cloning Molecular Aspergillus awamori Ethanol biology food and beverages General Medicine Starch analysis Recombinant Proteins Yeast Biochemistry chemistry Fermentation biology.protein Glucan 1 4-alpha-Glucosidase alpha-Amylases Alpha-amylase Biotechnology |
| Zdroj: | Biotechnology Letters. 33:1643-1648 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/s10529-011-0613-9 |
| Popis: | Industrial strains of a polyploid, distiller's Saccharomyces cerevisiae that produces glucoamylase and α-amylase was used for the direct fermentation of raw starch to ethanol. Strains contained either Aspergillus awamori glucoamylase gene (GA1), Debaryomyces occidentalis glucoamylase gene (GAM1) or D. occidentalis α-amylase gene (AMY), singly or in combination, integrated into their chromosomes. The strain expressing both GA1 and AMY generated 10.3% (v/v) ethanol (80.9 g l(-1)) from 20% (w/v) raw corn starch after 6 days of fermentation, and decreased the raw starch content to 21% of the initial concentration. |
| Databáze: | OpenAIRE |
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