Pyrosequencing the Midgut Transcriptome of the Banana Weevil Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae) Reveals Multiple Protease-Like Transcripts
| Autor: | Arnubio Valencia, Manuel Aristizábal, Jorge W. Arboleda, Blair D. Siegfried, Alberto Soto, Daniel D. Noriega, Seong-il Eyun, Haichuan Wang |
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| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
0301 basic medicine Insecticides Polymers lcsh:Medicine Chitin Bananas 01 natural sciences Biochemistry Transcriptome Bacillus thuringiensis lcsh:Science Phylogeny Genetics Multidisciplinary biology Weevil Gene Ontologies Insect physiology food and beverages Agriculture Genomics Proteases Plants Enzymes Chemistry Macromolecules Physical Sciences Insect Pests Sequence Analysis Transcriptome Analysis Research Article Sequence analysis Protein digestion Materials by Structure Materials Science Crops Insect Physiology Xenobiotics Fruits 03 medical and health sciences Pests Sequence Homology Nucleic Acid Botany Animals Animal Physiology RNA Messenger Invertebrate Physiology Gene Expression Profiling lcsh:R fungi Organisms Biology and Life Sciences Computational Biology Proteins Midgut biology.organism_classification Genome Analysis Polymer Chemistry 010602 entomology 030104 developmental biology Gene Ontology Proteolysis Enzymology Pyrosequencing Weevils lcsh:Q Digestive System Zoology Entomology Peptide Hydrolases Crop Science |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 3, p e0151001 (2016) |
| ISSN: | 1932-6203 |
| Popis: | The banana weevil Cosmopolites sordidus is an important and serious insect pest in most banana and plantain-growing areas of the world. In spite of the economic importance of this insect pest very little genomic and transcriptomic information exists for this species. In the present study, we characterized the midgut transcriptome of C. sordidus using massive 454-pyrosequencing. We generated over 590,000 sequencing reads that assembled into 30,840 contigs with more than 400 bp, representing a significant expansion of existing sequences available for this insect pest. Among them, 16,427 contigs contained one or more GO terms. In addition, 15,263 contigs were assigned an EC number. In-depth transcriptome analysis identified genes potentially involved in insecticide resistance, peritrophic membrane biosynthesis, immunity-related function and defense against pathogens, and Bacillus thuringiensis toxins binding proteins as well as multiple enzymes involved with protein digestion. This transcriptome will provide a valuable resource for understanding larval physiology and for identifying novel target sites and management approaches for this important insect pest. |
| Databáze: | OpenAIRE |
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