Pyrosequencing the Midgut Transcriptome of the Banana Weevil Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae) Reveals Multiple Protease-Like Transcripts

Autor: Arnubio Valencia, Manuel Aristizábal, Jorge W. Arboleda, Blair D. Siegfried, Alberto Soto, Daniel D. Noriega, Seong-il Eyun, Haichuan Wang
Rok vydání: 2015
Předmět:
0106 biological sciences
0301 basic medicine
Insecticides
Polymers
lcsh:Medicine
Chitin
Bananas
01 natural sciences
Biochemistry
Transcriptome
Bacillus thuringiensis
lcsh:Science
Phylogeny
Genetics
Multidisciplinary
biology
Weevil
Gene Ontologies
Insect physiology
food and beverages
Agriculture
Genomics
Proteases
Plants
Enzymes
Chemistry
Macromolecules
Physical Sciences
Insect Pests
Sequence Analysis
Transcriptome Analysis
Research Article
Sequence analysis
Protein digestion
Materials by Structure
Materials Science
Crops
Insect Physiology
Xenobiotics
Fruits
03 medical and health sciences
Pests
Sequence Homology
Nucleic Acid
Botany
Animals
Animal Physiology
RNA
Messenger
Invertebrate Physiology
Gene Expression Profiling
lcsh:R
fungi
Organisms
Biology and Life Sciences
Computational Biology
Proteins
Midgut
biology.organism_classification
Genome Analysis
Polymer Chemistry
010602 entomology
030104 developmental biology
Gene Ontology
Proteolysis
Enzymology
Pyrosequencing
Weevils
lcsh:Q
Digestive System
Zoology
Entomology
Peptide Hydrolases
Crop Science
Zdroj: PLoS ONE
PLoS ONE, Vol 11, Iss 3, p e0151001 (2016)
ISSN: 1932-6203
Popis: The banana weevil Cosmopolites sordidus is an important and serious insect pest in most banana and plantain-growing areas of the world. In spite of the economic importance of this insect pest very little genomic and transcriptomic information exists for this species. In the present study, we characterized the midgut transcriptome of C. sordidus using massive 454-pyrosequencing. We generated over 590,000 sequencing reads that assembled into 30,840 contigs with more than 400 bp, representing a significant expansion of existing sequences available for this insect pest. Among them, 16,427 contigs contained one or more GO terms. In addition, 15,263 contigs were assigned an EC number. In-depth transcriptome analysis identified genes potentially involved in insecticide resistance, peritrophic membrane biosynthesis, immunity-related function and defense against pathogens, and Bacillus thuringiensis toxins binding proteins as well as multiple enzymes involved with protein digestion. This transcriptome will provide a valuable resource for understanding larval physiology and for identifying novel target sites and management approaches for this important insect pest.
Databáze: OpenAIRE
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