Comparative study of the efficacy of pulsed electromagnetic field and low level laser therapy on mitogen-activated protein kinases
| Autor: | Mohammed H. Hassan, Alaa Eldin Balbaa, Radwa Mahmoud Elsharaby, Ayman M. El-Makakey |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway ERK Extracellular signal-Regulated Kinase medicine.medical_treatment p38 mitogen-activated protein kinases Biophysics DMEM Dulbecco's Modified Eagle Medium Pharmacology Pulsed electromagnetic field Biochemistry Flow cytometry lcsh:Biochemistry 03 medical and health sciences Extracellular Medicine lcsh:QD415-436 Mitogen-activated protein kinases lcsh:QH301-705.5 Low level laser therapy medicine.diagnostic_test Peripheral lymphocytes Kinase business.industry MAPKs Mitogen-Activated Protein Kinases LLLT Low Level Laser Therapy JNK C-JUN N-terminal Kinase 030104 developmental biology lcsh:Biology (General) Immunology PEMFT Pulsed Electromagnetic Field Therapy Signal transduction business Pulsed electromagnetic field therapy Research Article |
| Zdroj: | Biochemistry and Biophysics Reports Biochemistry and Biophysics Reports, Vol 9, Iss C, Pp 316-321 (2017) |
| ISSN: | 2405-5808 |
| DOI: | 10.1016/j.bbrep.2017.01.008 |
| Popis: | Mitogen-Activated Protein Kinases (MAPKs) consist of three major signaling members: extracellular signal-regulated kinase (ERK), p38 and C-JUN N-terminal kinase (JNK). We investigated physiological effects of Pulsed Electromagnetic Field Therapy (PEMFT) and Low Level Laser Therapy (LLLT) on human body, adopting the expression level of mitogen-activated protein kinases as an indicator via assessment of the activation levels of three major families of MAPKS, ERK, p38 and JNK in the peripheral lymphocytes of patients before and after the therapies. Assessment for the expression levels of MAPKs families' were done, in the peripheral lymphocytes of patients recently have appendectomy, using flow cytometric analysis of multiple signaling pathways, pre and post LLLT and PEMFT application (twice daily for 6 successive days) on the appendectomy wound. There were non-significant differences in the expression levels of MAPKs families' pre- therapies application. But there were significant increase in the ERK expression levels post application of LLLT compared to its pre application (p Highlights • PEMFT stimulates all pathways of MAPKs including ERK, P38 and C-Jun-terminal. • LLLT stimulates only the ERK pathway in MAPKS activation pathways. • PEMFT has a powerful healing effect more than LLLT as it increase the activation of ERK, P38 and C-Jun-N Terminal. • LLLT has more potent pain decreasing effect than PEMFT as it does not activate p38 pathway. • PEMFT should not be used as pain killer modality in acute pain but it's very useful to be used in chronic pain management with hypothalamic –ve Ingram due to its stimulatory effect on P38. |
| Databáze: | OpenAIRE |
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