Implication of Akt, ERK1/2 and alternative p38MAPK signalling pathways in human colon cancer cell apoptosis induced by green tea EGCG
| Autor: | M. J. Lorenzo, Ana Risco, Ana Cuenda, Alberto Álvarez-Barrientos, Francisco Centeno, Miguel A. Martín-Serrano, Rafal Zur, Maria Isabel Cerezo-Guisado |
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| Rok vydání: | 2015 |
| Předmět: |
MAPK/ERK pathway
Programmed cell death Food Handling MAP Kinase Signaling System Apoptosis Mitogen-activated protein kinase kinase Toxicology complex mixtures p38 Mitogen-Activated Protein Kinases Antioxidants Catechin chemistry.chemical_compound Cell Line Tumor Anticarcinogenic Agents Humans heterocyclic compounds Phosphorylation RNA Small Interfering Protein kinase A Protein kinase B Protein Kinase Inhibitors Tea Kinase Osmolar Concentration food and beverages General Medicine Isoenzymes HEK293 Cells chemistry Biochemistry Colonic Neoplasms Cancer research RNA Interference sense organs Protein Processing Post-Translational Proto-Oncogene Proteins c-akt Food Science |
| Zdroj: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 84 |
| ISSN: | 1873-6351 |
| Popis: | We investigated apoptosis induced by the green tea component the epigallocatechin-3-gallate (EGCG) and the pathways underlying its activity in a colon cancer cell line. A complete understanding of the mechanism(s) and molecules targeted by green tea polyphenols could be useful in developing novel therapeutic approaches for cancer treatment. EGCG, which is the major polyphenol in green tea, has cytotoxic effects and induced cell death in HT-29 cell death. In this study, we evaluated the effect EGCG on mitogen-activated protein kinase (MAPK) and Akt pathways. EGCG treatment increased phospho-ERK1/2, -JNK1/2 and -p38α, -p38γ and -p38δ, as well as phospho-Akt levels. Using a combination of kinase inhibitors, we found that EGCG-induced cell death is partially blocked by inhibiting Akt, ERK1/2 or alternative p38MAPK activity. Our data suggest that these kinase pathways are involved in the anti-cancer effects of EGCG and indicate potential use of this compound as chemotherapeutic agent for colon cancer treatment. |
| Databáze: | OpenAIRE |
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