Icariside II facilitates the differentiation of ADSCs to schwann cells and restores erectile dysfunction through regulation of miR-33/GDNF axis
| Autor: | Tao Zheng, Fan Yang, Tianbiao Zhang, Yangyang Sun, Wei-Xing Zhang, Kunlong Lv, Donghui Jia, Jing Lian, Rui Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine miR-33 Mean arterial pressure Erectile function Adipose tissue RM1-950 03 medical and health sciences 0302 clinical medicine Erectile Dysfunction Western blot Genes Reporter ADSCs Glial cell line-derived neurotrophic factor medicine Animals Glial Cell Line-Derived Neurotrophic Factor Viability assay Flavonoids Pharmacology biology medicine.diagnostic_test Chemistry Stem Cells Cell Differentiation General Medicine Nerve injury medicine.disease GDNF Rats Cell biology MicroRNAs 030104 developmental biology Erectile dysfunction Adipose Tissue Gene Expression Regulation nervous system 030220 oncology & carcinogenesis ICA II Differentiation biology.protein RNA Interference Schwann Cells Therapeutics. Pharmacology medicine.symptom Stem Cell Transplantation |
| Zdroj: | Biomedicine & Pharmacotherapy, Vol 125, Iss, Pp 109888-(2020) |
| ISSN: | 0753-3322 |
| Popis: | Background Adipose derived stem cells (ADSCs) have the property to differentiate into neuron-like cells, which may provide a novel insight for the restoration of erectile dysfunction (ED) mainly induced by cavernous nerve injury. Icariside II (ICA II) has been reported to play a key role in the regulation of erectile function via stimulating the differentiation of ADSCs to Schwann Cells (SCs). However, the function and molecular mechanisms of ICA II in ED remains to be further clarified. Methods The expression of S100, P75, GDNF and miR-33 was detected by qRT-PCR. And the relative proteins expression was determined by western blot. Cell viability was measured by Cell Counting Kit-8 (CCK-8) assay. Bioinformatics, luciferase reporter and RNA immunoprecipitation (RIP) assays were performed to verify the interaction between miR-33 and GDNF. Intracavernosal pressure (ICP), the ratio of ICP and mean arterial pressure (MAP), as well as nNOS expression were examined to evaluate the erectile function of SD rats with bilateral cavernous nerve injury (BCNI). Results ICA II and miR-33 respectively promoted and inhibited the differentiation of ADSCs to SCs. MiR-33 could negatively regulate P75 and GDNF expression. ICA II exerted promotion effects on differentiation of ADSCs to SCs via regulating miR-33. GDNF was identified to be a target of miR-33. MiR-33 overexpression abrogated the stimulatory effect of ICA II on ADSCs' differentiation, which was blocked by GDNF overexpression. treated with ICA II recovered the erectile function of BCNI model rats through regulation of miR-33. Conclusion ICA II contributed to the differentiation of ADSCs to SCs viamiR-33/GDNF axis, contributing to the recovery of erectile function in BCNI rats. |
| Databáze: | OpenAIRE |
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