Beta-carboline derivatives: novel photosensitizers that intercalate into DNA to cause direct DNA damage in photodynamic therapy
| Autor: | Xiaodong Liu, Yan Ma, Hongsheng Chen, Huaji Guan, Anlong Xu, Wenlie Peng, Rihui Cao |
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| Rok vydání: | 2006 |
| Předmět: |
Light
Cell Survival medicine.medical_treatment Biophysics Mitosis Photodynamic therapy Biology Biochemistry Absorption HeLa chemistry.chemical_compound Direct DNA damage Inhibitory Concentration 50 Necrosis medicine Humans Molecular Biology Cell Proliferation Cell Nucleus Photosensitizing Agents DNA synthesis Cell Death Cell growth Genome Human Cell Biology DNA biology.organism_classification Molecular biology Intercalating Agents Comet assay chemistry Photochemotherapy Apoptosis Carbolines DNA Damage HeLa Cells |
| Zdroj: | Biochemical and biophysical research communications. 342(3) |
| ISSN: | 0006-291X |
| Popis: | Novel 1,3,9-trisubstituted β-carboline derivatives were found to exhibit DNA photocleavage properties under visible light irradiation in a cell-free system, which could be reduced by antioxidant vitamin E. Their photo-cytotoxicity to human tumor cell line HeLa was confirmed, in which apoptosis only contributed a small part to the cell death, and necrosis was the dominating outcome of HeLa cells in photodynamic therapy (PDT) using β-carboline derivatives. Different from other clinical PDT drugs, β-carboline derivatives were demonstrated to be able to distribute in the nucleus and intercalate into DNA, and consequently cause direct DNA damage by photochemical reaction products in PDT, which was proved by the distinct DNA tails in the comet assay and the considerable amount of DNA damaged cells quantified by flow cytometry. This mechanism could be the explanation for the delay of cell proliferation at DNA synthesis and mitosis. |
| Databáze: | OpenAIRE |
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