Baicalin attenuates XRCC1-mediated DNA repair to enhance the sensitivity of lung cancer cells to cisplatin
| Autor: | Zhuo Cao, Zhangyong Yin, Xiaoping Cai, Enguo Chen, Zaiting Ye, Jiongwei Pan, Yuling Li, Enhui Gong, Cunlai Xu |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Lung Neoplasms DNA Repair DNA repair DNA damage Antineoplastic Agents Apoptosis Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor medicine Humans Cyclin D1 Viability assay Molecular Biology bcl-2-Associated X Protein Cisplatin Flavonoids Chemistry Cell Biology respiratory system Cell cycle respiratory tract diseases Comet assay 030104 developmental biology X-ray Repair Cross Complementing Protein 1 A549 Cells Drug Resistance Neoplasm 030220 oncology & carcinogenesis Cancer research Baicalin medicine.drug |
| Zdroj: | Journal of receptor and signal transduction research. 42(3) |
| ISSN: | 1532-4281 |
| Popis: | Baicalin plays important roles in different types of cancer. A previous report showed that baicalin attenuates cisplatin resistance in lung cancer. However, its mechanism remains unclear. In this study, we investigated the effect and mechanism of baicalin on DNA repair and sensitivity of lung cancer cells to cisplatin. A549 and A549/DPP cells were treated with baicalin and cisplatin. A549/DPP cells were transfected with XRCC1 and siXRCC1. Cell viability and DNA damage were detected by MTT and comet assay. Apoptosis rate and cell cycle were detected by flow cytometry assay. The expressions of Bax, Bcl-2, and Cyclin D1 were detected by western blot. XRCC1 expression was detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR) and western blot. Baicalin and cisplatin decreased cell viability in A549 and A549/DPP cells in dose-dependent manner. Baicalin enhanced the effect of cisplatin on promoting apoptosis, arresting cell on S stage and triggering DNA damage accompanied with the upregulation of Bcl-2-associated X protein (Bax) and downregulation of B-cell lymphoma 2 (Bcl-2) and Cyclin D1 in A549/DPP cells. Moreover, baicalin promoted the inhibitory effect of cisplatin on XRCC1 expression in A549 and A549/DPP cells. However, the synthetic effects of baicalin and cisplatin on A549/DPP cells were partially inhibited by XRCC1 overexpression and promoted by XRCC1 knockdown. This study demonstrates that baicalin interferes with XRCC1-mediated cellar DNA repair to sensitize lung cancer cells to cisplatin. |
| Databáze: | OpenAIRE |
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