Hyperforin Inhibits Cell Growth by Inducing Intrinsic and Extrinsic Apoptotic Pathways in Hepatocellular Carcinoma Cells
| Autor: | Bi-Jhih Chen, Mao-Chi Weng, Hwai-Jeng Lin, Yen-Chung Chen, Wei-Shu Wang, Chih-Wei Tseng, I-Tsang Chiang, Yu Cheng Kuo, Wei-Ting Chen |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Carcinoma Hepatocellular Time Factors Cell Survival Cell Apoptosis Cell Cycle Proteins Phloroglucinol Inhibitor of apoptosis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor medicine Humans MCL1 Viability assay Cell Proliferation Membrane Potential Mitochondrial Dose-Response Relationship Drug Terpenes Chemistry Cell growth Liver Neoplasms General Medicine Antineoplastic Agents Phytogenic G1 Phase Cell Cycle Checkpoints XIAP Cell biology Hyperforin 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Apoptosis Regulatory Proteins Signal Transduction |
| Zdroj: | Anticancer Research. 37:161-168 |
| ISSN: | 1791-7530 0250-7005 |
| DOI: | 10.21873/anticanres.11301 |
| Popis: | The aim of the present study was to investigate the antitumor effect and mechanism of action of hyperforin in hepatocellular carcinoma (HCC) SK-Hep1 cells in vitro. Cells were treated with different concentrations of hyperforin for different periods of time. Effects of hyperforin on cell viability, apoptosis signaling, and expression of anti-apoptotic and proliferative proteins [cellular FLICE-like inhibitory protein (c-FLIP), X-linked inhibitor of apoptosis protein (XIAP), myeloid cell leukemia 1(MCL1), and cyclin-D1] were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, and western blotting. Hyperforin significantly inhibited cell viability and expression of anti-apoptotic and proliferative proteins. We also found that hyperforin significantly induced accumulation of cells in sub-G1 phase, loss of mitochondrial membrane potential, and increased levels of active caspase-3, and caspase-8. Taken together, our findings indicate that hyperforin triggers inhibition of tumor cell growth by inducing intrinsic and extrinsic apoptotic pathways in HCC SK-Hep1 cells. |
| Databáze: | OpenAIRE |
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