CD4+T-cell activation is differentially modulated by bacteria-primed dendritic cells, but is generally down-regulated by n-3 polyunsaturated fatty acids
| Autor: | Pia Lund, Susanne Brix, Ellen Marie Straarup, Tanja Kjær, Hanne Frøkiær, Lars Hellgren |
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| Rok vydání: | 2010 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte CD4-Positive T-Lymphocytes Lipopolysaccharides CD3 Complex T cell Immunology chemical and pharmacologic phenomena Biology Lymphocyte Activation Inducible T-Cell Co-Stimulator Protein Interferon-gamma Mice Interleukin 21 CD28 Antigens Antigens CD Fatty Acids Omega-6 Fatty Acids Omega-3 medicine Animals Immunology and Allergy Cytotoxic T cell CTLA-4 Antigen Mesentery CD40 Antigens Cell Proliferation CD86 Antigen Presentation Mice Inbred BALB C CD40 Bacteria Fatty Acids Histocompatibility Antigens Class II Antibodies Monoclonal CD28 hemic and immune systems Dendritic Cells Original Articles Dendritic cell Dietary Fats Interleukin-10 Cell biology Intestines medicine.anatomical_structure B7-1 Antigen biology.protein B7-2 Antigen Lymph Nodes Spleen CD80 |
| Zdroj: | Pedersen, S B, Lund, P, Kjær, T, Straarup, E M, Hellgren, L & Frøkiær, H 2010, ' CD4+ T-cell activation is differentially modulated by bacteria-primed dendritic cells, but is generally down-regulated by n-3 polyunsaturated fatty acids ', Immunology, vol. 129, no. 3, pp. 338-350 . https://doi.org/10.1111/j.1365-2567.2009.03163.x |
| ISSN: | 1365-2567 0019-2805 |
| DOI: | 10.1111/j.1365-2567.2009.03163.x |
| Popis: | Appropriate activation of CD4(+) T cells is fundamental for efficient initiation and progression of acquired immune responses. Here, we showed that CD4(+) T-cell activation is dependent on changes in membrane n-3 polyunsaturated fatty acids (PUFAs) and is dynamically regulated by the type of signals provided by dendritic cells (DCs). Upon interaction with DCs primed by different concentrations and species of gut bacteria, CD4(+) T cells were activated according to the type of DC stimulus. The levels of CD80 were found to correlate to the levels of expression of CD28 and to the proliferation of CD4(+) T cells, while the presence of CD40 and CD86 on DCs inversely affected inducible costimulator (ICOS) and cytotoxic T-lymphocyte antigen-4 (CTLA-4) levels in CD4(+) T cells. For all DC stimuli, cells high in n-3 PUFAs showed reduced ability to respond to CD28 stimulation, to proliferate, and to express ICOS and CTLA-4. Diminished T-cell receptor (TCR) and CD28 signalling was found to be responsible for n-3 PUFA effects. Thus, the dietary fatty acid composition influences the overall level of CD4(+) T-cell activation induced by DCs, while the priming effect of the DC stimuli modulates CD80, CD86 and CD40 levels, thereby affecting and shaping activation of acquired immunity by differential regulation of proliferation and costimulatory molecule expression in CD4(+) T cells. |
| Databáze: | OpenAIRE |
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