Ethanol tolerance and membrane fatty acid adaptation in adh multiple and null mutants of Kluyveromyces lactis
| Autor: | Michele Saliola, S. Isken, Hermann J. Heipieper |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Membrane lipids
Phospholipid Microbiology chemistry.chemical_compound Kluyveromyces Microbiologie Molecular Biology Membrane adaptation Unsaturated fatty acid Alcohol dehydrogenase Kluyveromyces lactis chemistry.chemical_classification Ethanol biology Cell Membrane Fatty Acids Alcohol Dehydrogenase Fatty acid Fatty acid unsaturation General Medicine 1-Octanol biology.organism_classification Adh mutants ethanol tolerance Adaptation Physiological Yeast Culture Media Isoenzymes chemistry Biochemistry Mutation biology.protein |
| Zdroj: | Research in Microbiology, 151, 777-784 Research in Microbiology 151 (2000) |
| ISSN: | 0923-2508 |
| Popis: | The effects of ethanol and 1-octanol on growth and fatty acid composition of different strains of Kluyveromyces lactis containing a mutation in the four different alcohol dehydrogenase (KlADH) genes were investigated. In the presence of ethanol and 1-octanol K. lactis reduced the fluidity of its lipids by decreasing the unsaturation index (UI) of its membrane fatty acids. In this way, a direct correlation between nonlethal ethanol concentrations and the decrease in the UI could be observed. At concentrations which totally inhibited cell growth no reaction occurred. These adaptive modifications of the fatty acid pattern of K. lactis to ethanol contrasted with those reported for Saccharomyces cerevisiae and Schizosaccharomyces pombe. Whereas these two yeasts increased the fluidity of their membrane lipids in the presence of ethanol, K. lactis reduced the fluidity (UI) of its lipids. Among the different isogenic adh negative strains tested, the strain containing no ADH (adh0) and that containing only KlADH1 were the most alcohol-sensitive. The strain with only KlADH2 showed nearly the same tolerance as reference strain CBS 2359/152 containing all four ADH genes. This suggests that the KlADH2 product could play an important role in the adaptation/detoxification reactions of K. lactis to high ethanol concentrations. |
| Databáze: | OpenAIRE |
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