Induction of apoptosis by (-)-gossypol-enriched cottonseed oil in human breast cancer cells
| Autor: | Michael K. Dowd, Weiping Ye, Yasuro Sugimoto, Li-Shu Wang, Yi-Wen Huang, Sherry Shu, Young C. Lin, Peter J. Wan, Hsiang-Lin Chang |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Cottonseed Oil
Cell Blotting Western Apoptosis Breast Neoplasms DNA Fragmentation Biology Cell Line Tumor Genetics medicine Humans skin and connective tissue diseases Cells Cultured Cell Proliferation Electrophoresis Agar Gel Oncogene Dose-Response Relationship Drug Cell growth Reverse Transcriptase Polymerase Chain Reaction Gossypol Cancer Epithelial Cells General Medicine Cell cycle medicine.disease medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Cell culture Immunology Cancer cell Cancer research Female |
| Zdroj: | International journal of molecular medicine. 26(1) |
| ISSN: | 1791-244X |
| Popis: | Induction of apoptosis is one of the mechanisms of chemotherapeutic agents against breast cancer. In addition, recent studies have shown that diets containing polyphenolic components possess anticancer activities either in vitro or in vivo by inhibiting cell proliferation and inducing apoptosis. The aim of our study was to explore the effects of (-)-gossypol-enriched cottonseed oil [(-)-GPCSO], a polyphenolic compound, on the proliferation of the breast cancer cell line MCF-7 as well as primary cultured human breast cancer epithelial cells (PCHBCEC). We investigated whether the mechanism of the effects of (-)-GPCSO was mediated via the induction of cell apoptosis and the regulation of Bcl-2 gene expression at both the mRNA and protein levels. Our results showed that (-)-GPCSO inhibited the proliferation of MCF-7 and PCHBCEC in a dose-dependent manner. (-)-GPCSO (0.1 and 0.2%) induced DNA fragmentation in both MCF-7 cells and PCHBCEC. (-)-GPCSO suppressed the expression of Bcl-2 at both the mRNA and protein levels in MCF-7 cells and PCHBCEC in a dose-dependent fashion. Our results suggest that the growth inhibitory effect of (-)-GPCSO on MCF-7 and PCHBCEC is due, at least partially, to the induction of cell apoptosis, which is mediated by down-regulation of Bcl-2 expression at both the mRNA and protein levels. It might be possible for (-)-GPCSO to be developed as a novel chemotherapeutic agent for breast cancer patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|