Immunization against SIVmne in macaques using multigenic DNA vaccines
Autor: | William R. Morton, Robert J. Munn, Andrew Watson, Jannelle Thompson, Sally P. Mossman, LaRene Kuller, David C. Montefiori, Raoul E. Benveniste, Nancy L. Haigwood, Christopher C. Pierce, John B. Lynch, Philip D. Greenberg, Michael S. Rabin, Michael N. Robertson, Shiu Lok Hu |
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Rok vydání: | 1999 |
Předmět: |
Male
Genetic Vectors Simian Acquired Immunodeficiency Syndrome Priming (immunology) Biology Vaccines Attenuated Peripheral blood mononuclear cell Macaque Virus HIV Envelope Protein gp160 DNA vaccination law.invention Mice law biology.animal Vaccines DNA Animals Cloning Molecular AIDS Vaccines General Veterinary Immunogenicity T-Lymphocytes Helper-Inducer Viral Load Virology CD4 Lymphocyte Count Fusion Proteins gag-pol Macaca fascicularis DNA Viral biology.protein Recombinant DNA Immunization Simian Immunodeficiency Virus Animal Science and Zoology Antibody |
Zdroj: | Journal of Medical Primatology. 28:206-213 |
ISSN: | 0047-2565 |
DOI: | 10.1111/j.1600-0684.1999.tb00271.x |
Popis: | All structural and regulatory genes of SIVmne were cloned into mammalian expression vectors to optimize expression in vitro and immunogenicity in mice. Macaca fascicularis were immunized four times with plasmid DNA (n = 4), or two DNA priming inoculations followed by two boosts of recombinant gp160 plus Gag-Pol particles (n = 4). Following intrarectal challenge with SIVmne, all macaques became infected. Three monkeys immunized with DNA alone maintained low plasma virus loads by 1 year post-challenge; the fourth exhibited high virus loads and significant CD4 + cell decline. Two of the DNA plus boost and three control macaques had high virus loads and associated CD4 + cell decline. Both vaccine protocols elicited antibodies and comparable helper T-cell proliferative responses to gp160. Cytokine mRNA levels in activated peripheral blood mononuclear cells (PBMC) taken at time of challenge suggested a dominant T helper (Th) 1 state in three DNA-immunized and one protein-boosted macaque, which correlated with low virus loads and high CD4 + cell counts post-challenge. |
Databáze: | OpenAIRE |
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