Insights into eukaryotic multistep phosphorelay signal transduction revealed by the crystal structure of Ypd1p from Saccharomyces cerevisiae
| Autor: | Kyeongsik Min, Jinho Moon, Hyun Kyu Song, Se Won Suh, Myong Gyong Lee, Jin Kuk Yang, Jae Young Lee |
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| Rok vydání: | 1999 |
| Předmět: |
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Molecular Saccharomyces cerevisiae Proteins Recombinant Fusion Proteins Molecular Sequence Data Saccharomyces cerevisiae Crystallography X-Ray medicine.disease_cause Protein Structure Secondary Substrate Specificity Fungal Proteins Structure-Activity Relationship Bacterial Proteins Structural Biology Escherichia coli Slime mold medicine Transferase Histidine Amino Acid Sequence Phosphorylation Molecular Biology Conserved Sequence Helix bundle Binding Sites biology Escherichia coli Proteins Osmolar Concentration Phosphotransferases fungi Intracellular Signaling Peptides and Proteins Membrane Proteins biology.organism_classification Yeast DNA-Binding Proteins Biochemistry Signal transduction Crystallization Protein Kinases Signal Transduction |
| Zdroj: | Journal of Molecular Biology. 293:753-761 |
| ISSN: | 0022-2836 |
| DOI: | 10.1006/jmbi.1999.3215 |
| Popis: | “Two-component” phosphorelay signal transduction systems constitute a potential target for antibacterial and antifungal agents, since they are found exclusively in prokaryotes and lower eukaryotes (yeast, fungi, slime mold, and plants) but not in mammalian organisms. Saccharomyces cerevisiae Ypd1p, a key intermediate in the osmosensing multistep phosphorelay signal transduction, catalyzes the phosphoryl group transfer between response regulators. Its 1.8 A structure, representing the first example of a eukaryotic phosphorelay protein, contains a four-helix bundle as in the HPt domain of Escherichia coli ArcB sensor kinase. However, Ypd1p has a 44-residue insertion between the last two helices of the helix bundle. The side-chain of His64, the site of phosphorylation, protrudes into the solvent. The structural resemblance between Ypd1p and ArcB HPt domain suggests that both prokaryotes and lower eukaryotes utilize the same basic protein fold for phosphorelay signal transduction. This study sheds light on the best characterized eukaryotic phosphorelay system. |
| Databáze: | OpenAIRE |
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