Amelioration of Experimentally Induced Arthritis by Reducing Reactive Oxygen Species Production through the Intra-Articular Injection of Water-Soluble Fullerenol
| Autor: | Edmund Cheung So, Jian Hua Chen, Wen Shuo Kuo, Chao Liang Wu, Shih Yao Chen, Po-Ting Wu, Chia Tse Weng, Ai Li Shiau, Jeng Long Hsieh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
musculoskeletal diseases
rheumatoid arthritis Angiogenesis General Chemical Engineering medicine.medical_treatment Arthritis 02 engineering and technology Pharmacology Article Nitric oxide lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound medicine water-soluble fullerenol General Materials Science 030304 developmental biology intra-articular injection chemistry.chemical_classification reactive oxygen species 0303 health sciences Reactive oxygen species Interleukin 021001 nanoscience & nanotechnology medicine.disease experimental arthritis Vascular endothelial growth factor Cytokine lcsh:QD1-999 chemistry Rheumatoid arthritis 0210 nano-technology |
| Zdroj: | Nanomaterials Volume 9 Issue 6 Nanomaterials, Vol 9, Iss 6, p 909 (2019) |
| ISSN: | 2079-4991 |
| DOI: | 10.3390/nano9060909 |
| Popis: | Accumulated evidence suggests a pathogenic role of reactive oxygen species (ROS) in perpetually rheumatoid joints. Therefore, the application of radical scavengers for reducing the accumulation of ROS is beneficial for patients with rheumatoid arthritis (RA). We synthesized water-soluble fullerenols that could inhibit the production of ROS and applied intra-articular (i.a.) injection in an experimental arthritis model to examine the anti-arthritic effect of the synthesized compound. RAW 264.7 cells were used to examine the activity of the synthesized fullerenol. Collagen-induced arthritis (CIA) was induced in Sprague&ndash Dawley rats by injecting their joints with fullerenol. The therapeutic effects were evaluated using the articular index as well as radiological and histological scores. Dose-dependent suppression of nitric oxide (NO) production caused by the fullerenol was demonstrated in the RAW 264.7 cell culture, thus confirming the ability of fullerenol to reduce ROS production. In the fullerenol-injected joints, articular indexes, synovial expression of ROS, histological and radiological scores, pannus formation, and erosion of cartilage and bone were all reduced. Moreover, interleukin (IL)-1&beta and vascular endothelial growth factor (VEGF) levels were reduced, and fewer von Willebrand factor (vWF)-stained areas were identified in the fullerenol-treated joints than in control joints. The i.a. injection of fullerenol for reducing ROS production can ameliorate arthritis in joints by suppressing pro-inflammatory cytokine production and the angiogenesis process. Thus, the i.a. injection of fullerenol for reducing the production of ROS can be used as a pharmacological approach for RA patients. |
| Databáze: | OpenAIRE |
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