Receptors for anti-Müllerian hormone on Leydig cells are responsible for its effects on steroidogenesis and cell differentiation
| Autor: | Ilpo Huhtaniemi, F. Louis, Rodolfo Rey, A Ferré, Maguelone G. Forest, Nathalie Josso, N di Clemente, Chrystèle Racine |
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| Rok vydání: | 1998 |
| Předmět: |
Anti-Mullerian Hormone
Male endocrine system medicine.medical_specialty Receptors Peptide endocrine system diseases Cellular differentiation Transgene Mice Transgenic Mice Internal medicine medicine Animals Receptor Cells Cultured Glycoproteins Multidisciplinary biology Leydig cell urogenital system luteinizing hormone/choriogonadotropin receptor Gene Transfer Techniques Leydig Cells Cell Differentiation Anti-Müllerian hormone Leydig cell differentiation Biological Sciences female genital diseases and pregnancy complications Growth Inhibitors Testicular Hormones Endocrinology medicine.anatomical_structure biology.protein Steroids Signal transduction Receptors Transforming Growth Factor beta hormones hormone substitutes and hormone antagonists Signal Transduction |
| Zdroj: | Proceedings of the National Academy of Sciences. 95:594-599 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.95.2.594 |
| Popis: | Strong overexpression of anti-Müllerian hormone (AMH) in transgenic mice leads to incomplete fetal virilization and decreased serum testosterone in the adult. Conversely, AMH-deficient mice exhibit Leydig cell hyperplasia. To probe the mechanism of action of AMH on Leydig cell steroidogenesis, we have studied the expression of mRNA for steroidogenic proteins in vivo and in vitro and performed a morphometric analysis of testicular tissue in mice overexpressing the hormone. We show that overexpression of AMH in male transgenic mice blocks the differentiation of Leydig cell precursors. Expression of steroidogenic protein mRNAs, mainly cytochrome P450 17α-hydroxylase/C17–20 lyase (P450c17), is decreased in transgenic mice overexpressing AMH and in AMH-treated purified Leydig cells. In contrast, transgenic mice in whom the AMH locus has been disrupted show increased expression of P450c17. In vitro , but not in vivo , AMH also decreases the expression of the luteinizing hormone receptor. The effect of AMH is explained by the presence of its receptor on Leydig cells. Our results provide insight into the action of AMH as a negative modulator of Leydig cell differentiation and function. |
| Databáze: | OpenAIRE |
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