In-Depth Genetic Diversity and Population Structure of Endangered Peruvian Amazon Rosewood Germplasm Using Genotyping by Sequencing (GBS) Technology
| Autor: | Juan Carlos Castro Gómez, Muhammad Amjad Nawaz, Ephrem Habyarimana, Fawad Ali, Seung Hwan Yang, Esperanza Torres Canales, Muhammad Azhar Nadeem, Sezai Ercisli, Muhammad Qasim Shahid, Muhammad Aasim, Pedro Adrianzen Julca, Gyuhwa Chung, Tolga Karaköy, Jorge Luis Marapara Del Aguila, Faheem Shehzad Baloch, Stalin Juan Vasquez Guizado, Rüştü Hatipoğlu |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Germplasm molecular markers Population Biodiversity Endangered species Population genetics germplasm characterization Biology 010603 evolutionary biology 01 natural sciences Analysis of molecular variance 03 medical and health sciences education Aniba rosaeodora 030304 developmental biology 0303 health sciences Genetic diversity education.field_of_study DArTseq population genetics Forestry lcsh:QK900-989 Rosewood Evolutionary biology lcsh:Plant ecology |
| Zdroj: | Forests Volume 12 Issue 2 Forests, Vol 12, Iss 197, p 197 (2021) |
| ISSN: | 1999-4907 |
| DOI: | 10.3390/f12020197 |
| Popis: | Research studies on conservative genetics of endangered plants are very important to establish the management plans for the conservation of biodiversity. Rosewood is an evergreen tree of the Amazon region and its essential oil has great acceptance in the medical and cosmetic industry. The present study aimed to explore the genetic diversity and population structure of 90 rosewood accessions collected from eight localities of Peruvian Amazon territory through DArTseq markers. A total of 7485 informative markers resulted from genotyping by sequencing (GBS) analysis were used for the molecular characterization of rosewood germplasm. Mean values of various calculated diversity parameters like observed number of alleles (1.962), the effective number of alleles (1.669), unbiased expected heterozygosity (0.411), and percent polymorphism (93.51%) over the entire germplasm showed the existence of a good level of genetic variations. Our results showed that the Mairiricay population was more diverse compared to the rest of the populations. Tamshiyacu-2 and Mairiricay-15 accessions were found genetically distinct accessions. The analysis of molecular variance (AMOVA) reflected maximum variations (75%) are due to differences within populations. The implemented clustering algorithms, i.e., STRUCTURE, neighbor-joining analysis and principal coordinate analysis (PCoA) separated the studied germplasm on the basis of their geographical locations. Diversity indices for STRUCTURE-based populations showed that subpopulation A is more diverse population than the rest of the populations, for such reason, individuals belonging to this subpopulation should be used for reintroduction or reinforcement plans of rosewood conservation. We envisage that molecular characterization of Peruvian rosewood germplasm with DArTseq markers will provide a platform for the conservation, management and restoration of endangered rosewood in upcoming years. |
| Databáze: | OpenAIRE |
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