Generation of six human induced pluripotent stem cell sublines (MZT01E, MZT01F, MZT01N and MZT02D, MZT02G and MZT02H) for reproductive science research
| Autor: | Enrique Sosa, Sherman J. Silber, Timothy J. Hunt, Erica C. Pandolfi, Sierra Goldsmith, Amander T. Clark, Kellie Hurlbut |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Homeobox protein NANOG Induced Pluripotent Stem Cells Monozygotic twin Regenerative Medicine Sendai virus Medical and Health Sciences Article 03 medical and health sciences 0302 clinical medicine Rare Diseases Clinical Research Humans Induced pluripotent stem cell lcsh:QH301-705.5 Skin biology Stem Cell Research - Induced Pluripotent Stem Cell - Human Stem Cell Research - Induced Pluripotent Stem Cell Contraception/Reproduction Ovarian failure Cell Differentiation Cell Biology General Medicine Fibroblasts Biological Sciences biology.organism_classification Cellular Reprogramming Stem Cell Research 030104 developmental biology Science research lcsh:Biology (General) embryonic structures Cancer research Female 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 51, Iss, Pp 102204-(2021) Stem Cell Res |
| ISSN: | 1873-5061 |
| Popis: | Six human induced pluripotent stem cell sublines (hiPSCs) were generated from human dermal fibroblasts (HDFs) derived from skin biopsies donated from monozygotic twin women wherein one woman had proven fertility and her sister was infertile due to ovarian failure. Three hiPSC sublines were created from each twin's HDFs. hiPSCs were reprogrammed using Sendai virus vectors and were subsequently positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA-4. Pluripotency was further verified using PluriTest. We show here that the hiPSC lines created from the twins are equivalent in measures of pluripotency and self-renewal, despite their differential diagnosis. |
| Databáze: | OpenAIRE |
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