Plasma and urine leukocyte elastase- 1protease inhibitor complex as a marker of early and long-term kidney graft function
| Autor: | Marian Klinger, Przemyslaw Biecek, Dariusz Patrzałek, Wacław Kopeć, Zofia Marchewka, Jakub Kuźniar, Mariusz Kusztal, M Zynek-Litwin |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male medicine.medical_specialty medicine.medical_treatment Urology Urine Neutrophil Activation Excretion Predictive Value of Tests medicine Humans Protease Inhibitors Kidney transplantation Aged Retrospective Studies Transplantation Kidney business.industry Graft Survival Middle Aged medicine.disease Kidney Transplantation medicine.anatomical_structure Nephrology alpha 1-Antitrypsin Multivariate Analysis Immunology Neutrophil degranulation Female Hemodialysis Leukocyte Elastase business Biomarkers Kidney disease |
| Zdroj: | Nephrology Dialysis Transplantation. 25:2346-2351 |
| ISSN: | 1460-2385 0931-0509 |
| DOI: | 10.1093/ndt/gfq041 |
| Popis: | Neutrophils are mediators of ischaemia/reperfusion (I/R) injury following kidney transplantation (kTx). Leukocyte elastase (LE) complex with alpha(1)protease inhibitor (LE-alpha(1)PI) is a marker of neutrophil degranulation. The aim of this study was to evaluate LE-alpha(1)PI as a marker of I/R kidney damage and to search for correlations between leukocyte activation and post-transplant complications.Plasma and urine LE-alpha(1)PI were estimated in 55 deceased-donor kidney graft recipients on postoperative days (POD) 1, 3 and 7, as well as in the late post-transplant period.The plasma LE-alpha(1)PI level peaked on POD 1 after kTx, and the urine LE-alpha(1)PI peaked on POD 3. On POD 1 and POD 3, the urine LE-alpha(1)PI levels were higher in delayed graft function (DGF) patients than in patients with immediate graft function (IGF: P0.001 and P0.003, respectively). Urine LE-alpha(1)PI excretion on POD 1 was significantly higher in patients with longer cold ischaemia time (CIT) than in patients with shorter CIT, P0.002. Multivariate regression model revealed two factors influencing the occurrence of early acute rejection-urine LE-alpha(1)PI complex on POD 3 and human leukocyte antigen (HLA) mismatches. There was a significant association between the plasma LE-alpha(1)PI on POD 3 and serum creatinine level 6 and 12 months after kTx (r(2) 0.24; P0.005 and 0.19; P0.005, respectively).This study is the first presentation of a simple, non-invasive measurement of neutrophil activation after kTx. It also demonstrates a strong correlation between the early post-transplant LE-alpha(1)PI complex level and kidney graft function. |
| Databáze: | OpenAIRE |
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