Molecular Cloning and Expression of a cDNA Encoding a Hybrid Histidine Kinase Receptor in Tropical Periwinkle Catharanthus roseus
| Autor: | Nicolas Papon, Jennifer Bremer, Joël Crèche, Marc Rideau, Gaëlle Glévarec, Amerin Vansiri |
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| Rok vydání: | 2006 |
| Předmět: |
DNA
Complementary DNA Plant Histidine Kinase Catharanthus Molecular Sequence Data Receptors Cell Surface Plant Science Molecular cloning Gene Expression Regulation Enzymologic Homology (biology) Gene Expression Regulation Plant Arabidopsis Complementary DNA Cloning Molecular Gene Peptide sequence Phylogeny Ecology Evolution Behavior and Systematics Plant Proteins biology Histidine kinase General Medicine Catharanthus roseus biology.organism_classification Biochemistry Protein Kinases |
| Zdroj: | Plant Biology. 8:731-736 |
| ISSN: | 1438-8677 1435-8603 |
| Popis: | Signalling pathways involving histidine kinase receptors (HKRs) are widely used by prokaryotes and fungi to regulate a large palette of biological processes. In plants, HKRs are known to be implicated in cytokinin, ethylene, and osmosensing transduction pathways. In this work, a full length cDNA named CrCIK was isolated from the tropical species Catharanthus roseus (L.) G. Don. It encodes a 1205 amino acid protein that belongs to the hybrid HKR family. The deduced amino acid sequence shows the highest homology with AtHK1, an osmosensing HKR in Arabidopsis thaliana. In return, CrCIK protein shares very low identity with the other 10 Arabidopsis HKRs. Southern blot analysis indicates that the CrCIK corresponding gene is either present in multiple copies or has very close homologues in the genome of the tropical periwinkle. The gene is widely expressed in the plant. In C. roseus C20D cell suspension, it is slightly induced after exposure to low temperature, pointing to a putative role in cold-shock signal transduction. |
| Databáze: | OpenAIRE |
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