Point-of-care measurements reveal release of purines into venous blood of stroke patients
| Autor: | Ravjit Sagoo, Norman Phillips, Nicholas Dale, Christopher H.E. Imray, Faming Tian, Christine Roffe |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Purine Adult Male medicine.medical_specialty medicine.medical_treatment Point-of-Care Systems Biosensing Techniques 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 0302 clinical medicine Internal medicine Medicine Humans Prospective Studies cardiovascular diseases Purine metabolism Molecular Biology Stroke Cause of death Point of care Aged Aged 80 and over business.industry Cell Biology Venous blood Thrombolysis Cerebral ischemia Middle Aged RC346 medicine.disease R1 030104 developmental biology Clinical research chemistry Purines Point-of-Care Testing Cardiology Original Article Female business 030217 neurology & neurosurgery Biosensor RC |
| Zdroj: | Purinergic Signalling |
| ISSN: | 1573-9538 |
| Popis: | Stroke is a leading cause of death and disability. Here, we examine whether point-of-care measurement of the purines, adenosine, inosine and hypoxanthine, which are downstream metabolites of ATP, has potential to assist the diagnosis of stroke. In a prospective observational study, patients who were suspected of having had a stroke, within 4.5 h of symptom onset and still displaying focal neurological symptoms at admission, were recruited. Clinical research staff in the Emergency Departments of two hospitals used a prototype biosensor array, SMARTCap, to measure the purines in the venous blood of stroke patients and healthy controls. In controls, the baseline purines were 7.1 ± (SD) 4.2 μM (n = 52), while in stroke patients, they were 11.6 ± 8.9 μM (n = 76). Using the National Institutes for Stoke Scale (NIHSS) to band the severity of stroke, we found that minor, moderate and severe strokes all gave significant elevation of blood purines above the controls. The purine levels fall over 24 h. This was most marked for patients with haemorrhagic strokes (5.1 ± 3.6 μM, n = 9 after 24 h). The purine levels measured on admission show a significant correlation with the volume of affected brain tissue determined by medical imaging in patients who had not received thrombolysis or mechanical thrombectomy. ClinicalTrials.gov Identifier: NCT02308605 |
| Databáze: | OpenAIRE |
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