Induction of apoptosis in human endothelial cells by nanodiamond particles
| Autor: | K. Solarska, Agnieszka Gajewska, Grzegorz Bartosz, Katarzyna Mitura |
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| Rok vydání: | 2012 |
| Předmět: |
Materials science
Biocompatibility Dose-Response Relationship Drug Acridine orange Biomedical Engineering Nanoparticle Endothelial Cells Bioengineering Apoptosis General Chemistry Condensed Matter Physics Cell Line chemistry.chemical_compound chemistry Cell culture Annexin Materials Testing Biophysics Humans Nanoparticles General Materials Science Propidium iodide Diamond Cytotoxicity |
| Zdroj: | Journal of nanoscience and nanotechnology. 12(6) |
| ISSN: | 1533-4880 |
| Popis: | Carbon nanoparticles are a promising material which finds application in different fields in industry and medicine. For medical applications, biocompatibility of nanoparticles is of critical importance because a lot of medical implants are coated by carbon coating. Our previous results showed that nanoparticles may induce increased production of ROS by the cells so we decided to checked if nanopowders can induce apoptosis. Apoptosis was quantified by double-staining with acridine orange and ethidium bromide. For comparison, we identified apoptotic cells with annexin V-FITC/propidium iodide. Our data demonstrate that treatment of the cells with diamond nanopowders may induce apoptosis and necrosis and this effect is dependent on the time of treatment and concentration of the nanopowders. The highest level of apoptotic cells was observed after incubation with Ultrananocrystalline Detonation Diamond (UDD) suggesting that the size is the main determinant of nanoparticle cytotoxicity. |
| Databáze: | OpenAIRE |
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