Hahb-10, a Sunflower Homeobox-Leucine Zipper Gene, is Regulated by Light Quality and Quantity, and Promotes Early Flowering when Expressed in Arabidopsis
| Autor: | Eva Carolina Rueda, Carlos A. Dezar, Raquel Lia Chan, Daniel H. Gonzalez |
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| Rok vydání: | 2005 |
| Předmět: |
Leucine zipper
HAHB-10 DNA Plant Light Physiology Arabidopsis Plant Science Genetically modified crops SUNFLOWER HOMEODOMAIN-LEUCINE ZIPPER Biology Genes Plant purl.org/becyt/ford/1 [https] Ciencias Biológicas Genética y Herencia Gene Expression Regulation Plant Botany Helianthus annuus purl.org/becyt/ford/1.6 [https] Flowering Tops Gene Plant Proteins GIBBERELLIN Leucine Zippers LIGHT-REGULATED EXPRESSION Plant Stems Genes Homeobox food and beverages PSBS Cell Biology General Medicine Blotting Northern Plants Genetically Modified biology.organism_classification Gibberellins Plant Leaves HD-ZIP Phenotype RNA Plant Etiolation Helianthus Gibberellin Cauliflower mosaic virus CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 1471-9053 0032-0781 |
| DOI: | 10.1093/pcp/pci210 |
| Popis: | Homeodomain-leucine zipper proteins constitute a family of transcription factors found only in plants. Expression patterns of the sunflower homeobox-leucine zipper gene Hahb-10 (Helianthus annuus homeobox-10), that belongs to the HD-Zip II subfamily, were analysed. Northern blots showed that Hahb-10 is expressed primarily in mature leaves, although expression is clearly detectable in younger leaves and also in stems. Considerably higher expression levels were detected in etiolated seedlings compared with light-grown seedlings. Induction of Hahb-10 expression was observed when seedlings were subjected to treatment with gibberellins. Transgenic Arabidopsis thaliana plants that express Hahb-10 under the 35S cauliflower mosaic virus promoter show special phenotypic characteristics such as darker cotyledons and planar leaves. A reduction in the life cycle of about 25% allowing earlier seed collection was also observed, and this phenomenon is clearly related to a shortened flowering time. When the number of plants per pot increased, the difference in developmental rate between transgenic and non-transformed individuals became larger. After gibberellin treatment, the relative difference in life cycle duration was considerably reduced. Several light-regulated genes have been tested as possible target genes of Hahb-10. One of them, PsbS, shows a different response to illumination conditions in transgenic plants compared with the response in wild-type plants while the other genes behave similarly in both genotypes. We propose that Hahb-10 functions in a signalling cascade(s) that control(s) plant responses to light quality and quantity, and may also be involved in gibberellin transduction pathways. Fil: Rueda, Eva Carolina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Dezar, Carlos Alberto Alejandro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Gonzalez, Daniel Hector. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Chan, Raquel Lia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina |
| Databáze: | OpenAIRE |
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