Gene expression analysis reveals novel altered genes in nasal polyps
| Autor: | Janes Lopes Bonilha, Paula Rahal, Paola Jocelan Scarin Provazzi, Erica Babeto, Marilia Freitas Calmon, Atílio Maximino Fernandes, José Antônio Cordeiro, Jucimara Colombo |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Faculdade de Medicina de São José do Rio Preto (FAMERP) |
| Rok vydání: | 2010 |
| Předmět: |
Cancer Research
NOVA1 Population Mucous membrane of nose Biology Biochemistry Exon Gene expression Genetics medicine SOLH Nasal polyps education Molecular Biology Gene education.field_of_study nasal polyps methylated CpG island amplification ATP2A1 medicine.disease Molecular biology Real-time polymerase chain reaction Oncology CpG site PLCD3 Cancer research Molecular Medicine TGF beta 1 Real-time PCR |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr_00000285 |
| Popis: | Made available in DSpace on 2013-08-12T18:23:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-05-01 Made available in DSpace on 2013-09-30T18:41:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-05-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:00:54Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T14:00:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-05-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Nasal polyposis (NP) is a chronic inflammatory disease of the nasal mucosa characterized by the infiltration of inflammatory cells, mainly eosinophils. Although nasal polyposis occurs in 4% of the population, its physiopathology remains unclear. The aim of this study was to identify and characterize differentially expressed genes that can be used in the prognosis: treatment and elucidation of this physiopathology. To identify novel genes differentially expressed in NP, we applied real-time quantitative PCR to 11 NP samples and to a pool of total RNA from a subset of 13 normal nasal mucosa samples from human autopsies. For selecting genes, the methylated CpG island amplification technique was used. Five differentially methylated clones (ATP2A1, NOVA1, PLCD3, SOLH and TGF beta 1) were identified. However, these genes presented methylated CpG islands between exons, i.e., not in the promoter regions of the genes. Thus, as shown by real-time PCR, the ATP2A1, SOLH, PLDC3 and TGF beta 1 genes were overexpressed in NP. The genes identified in this study are probably involved in some stage of the process of formation and development of nasal polyposis, as they were highly expressed in the nasal polyp samples. UNESP, IBILCE, Dept Biol, Lab Genom Studies, BR-15054000 Sao Jose do Rio Preto, SP, Brazil Sao Jose do Rio Preto Sch Med FAMERP, Coll Med, Dept Epidemiol & Collect Hlth, Sao Jose do Rio Preto, SP, Brazil Sao Jose do Rio Preto Sch Med FAMERP, Coll Med, Dept Pathol, Sao Jose do Rio Preto, SP, Brazil Sao Jose do Rio Preto Sch Med FAMERP, Coll Med, Dept Otorhinolaryngol Head & Neck Surg, Sao Jose do Rio Preto, SP, Brazil UNESP, IBILCE, Dept Biol, Lab Genom Studies, BR-15054000 Sao Jose do Rio Preto, SP, Brazil |
| Databáze: | OpenAIRE |
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