Concurrent host-pathogen gene expression in the lungs of pigs challenged with Actinobacillus pleuropneumoniae
| Autor: | Kirstine Klitgaard, Tim Kåre Jensen, Peter M. H. Heegaard, Mette Sif Hansen, Kerstin Skovgaard, Louise Brogaard |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
36002
Medical and clinical microbiology - Bacteriology Medical Sciences Swine virulence factors 03502 Genetics - General iron 7439-89-6 metabolism pig TLR4 gene [Suidae] expression laser capture microdissection laboratory techniques histology and cytology techniques interleukin-1B IL-1B proinflammatory cytokine EXPERIMENTAL-INFECTION Actinobacillus Infections interleukin-6 IL-6 proinflammatory cytokine Respiratory infection histopathological examination laboratory techniques histology and cytology techniques 10069 Biochemistry studies - Minerals BIOTECHNOLOGY high-throughput RT-qPCR laboratory techniques genetic techniques Pathogen Lung Regulation of gene expression Swine Diseases 16006 Respiratory system - Pathology Innate immunity Pleuropneumonia biology INDUCTION IRON Actinobacillus pleuropneumoniae pig MD2 gene [Suidae] expression lipoprotein synthesis Laser capture microdissection RNA Bacterial lipopolysaccharide synthesis 10068 Biochemistry studies - Carbohydrates pleuropneumonia Pleuropneumonia (MeSH) respiratory system disease pathology mortality Infection exotoxins Research Article Artiodactyla Mammalia Vertebrata Chordata Animalia (Animals Artiodactyls Chordates Mammals Nonhuman Vertebrates Nonhuman Mammals Vertebrates) - Suidae [85740] pig common host commercial species pig CD14 gene [Suidae] expression Transcriptional analysis Biotechnology Veterinary Medicine medicine.medical_specialty ACUTE-PHASE RESPONSE GENETICS Virulence Factors PROTEINS mRNA 38002 Veterinary science - General and methods Virulence High-throughput RT-qPCR Microbiology 16004 Respiratory system - Physiology and biochemistry Molecular Genetics Bacterial Proteins Molecular genetics 03506 Genetics - Animal interleukin-8 IL-8 proinflammatory cytokine medicine Genetics Animals 10062 Biochemistry studies - Nucleic acids purines and pyrimidines peptidoglycan synthesis GRAM-NEGATIVE BACTERIA 17002 Endocrine - General lungs respiratory system Innate immune system 10064 Biochemistry studies - Proteins peptides and amino acids pathogen-associated molecular patterns Biochemistry and Molecular Biophysics 12502 Pathology - General IDENTIFICATION RECEPTOR Host-pathogen interactions pig LBP gene [Suidae] expression Gene Expression Profiling 31500 Genetics of bacteria and viruses Gene Expression Regulation Bacterial biology.organism_classification 38004 Veterinary science - Pathology PULMONARY-LESIONS Gene expression profiling pig MYD88 gene [Suidae] expression 31000 Physiology and biochemistry of bacteria Immunology RNA 10066 Biochemistry studies - Lipids Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria Eubacteria Microorganisms) - Pasteurellaceae [06703] Actinobacillus pleuropneumoniae species pathogen |
| Zdroj: | Brogaard, L, Schou, K K, Heegaard, P M H, Hansen, M S, Jensen, T K & Skovgaard, K 2015, ' Concurrent host-pathogen gene expression in the lungs of pigs challenged with Actinobacillus pleuropneumoniae ', B M C Genomics, vol. 16, no. 417 . https://doi.org/10.1186/s12864-015-1557-6 BMC Genomics |
| ISSN: | 1471-2164 |
| DOI: | 10.1186/s12864-015-1557-6 |
| Popis: | Background Actinobacillus pleuropneumoniae causes pleuropneumonia in pigs, a disease which is associated with high morbidity and mortality, as well as impaired animal welfare. To obtain in-depth understanding of this infection, the interplay between virulence factors of the pathogen and defense mechanisms of the porcine host needs to be elucidated. However, research has traditionally focused on either bacteriology or immunology; an unbiased picture of the transcriptional responses can be obtained by investigating both organisms in the same biological sample. Results Host and pathogen responses in pigs experimentally infected with A. pleuropneumoniae were analyzed by high-throughput RT-qPCR. This approach allowed concurrent analysis of selected genes encoding proteins known or hypothesized to be important in the acute phase of this infection. The expression of 17 bacterial and 31 porcine genes was quantified in lung samples obtained within the first 48 hours of infection. This provided novel insight into the early time course of bacterial genes involved in synthesis of pathogen-associated molecular patterns (lipopolysaccharide, peptidoglycan, lipoprotein) and genes involved in pattern recognition (TLR4, CD14, MD2, LBP, MYD88) in response to A. pleuropneumoniae. Significant up-regulation of proinflammatory cytokines such as IL1B, IL6, and IL8 was observed, correlating with protein levels, infection status and histopathological findings. Host genes encoding proteins involved in iron metabolism, as well as bacterial genes encoding exotoxins, proteins involved in adhesion, and iron acquisition were found to be differentially expressed according to disease progression. By applying laser capture microdissection, porcine expression of selected genes could be confirmed in the immediate surroundings of the invading pathogen. Conclusions Microbial pathogenesis is the product of interactions between host and pathogen. Our results demonstrate the applicability of high-throughput RT-qPCR for the elucidation of dual-organism gene expression analysis during infection. We showed differential expression of 12 bacterial and 24 porcine genes during infection and significant correlation of porcine and bacterial gene expression. This is the first study investigating the concurrent transcriptional response of both bacteria and host at the site of infection during porcine respiratory infection. Electronic supplementary material The online version of this article (doi:10.1186/s12864-015-1557-6) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|