Kinetics of inhibitory effect of calix[4]arene С-90 on activity of transporting plasma membrane Cа(2+),Mg(2+)-ATPase of smooth muscle cells
Autor: | Roman V. Rodik, Vitaly I. Kalchenko, Kosterin So, Veklich To, Iu Iu Mazur, Shkrabak Oa |
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Rok vydání: | 2014 |
Předmět: |
calix[4]arenes
chemistry.chemical_classification kinetic properties of ATPase Magnesium Stereochemistry myometrium Kinetics Myometrium enzymatic hydrolysis of ATP chemistry.chemical_element plasma membrane Biochemistry smooth muscle cells Divalent lcsh:Biochemistry Ca2+ chemistry.chemical_compound Membrane Digitonin chemistry Mg(2+)-ATPase ATP hydrolysis lcsh:QD415-436 Uncompetitive inhibitor |
Zdroj: | Ukrainian Biochemical Journal, Vol 86, Iss 5, Pp 37-46 (2014) |
ISSN: | 2409-4943 |
DOI: | 10.15407/ubj86.05.037 |
Popis: | In experiments on the suspension of myometrium cell plasma membrane, processed by 0.1% digitonin, the inhibitory action of calix[4]arene C-90 (5,11,17,23-tetra(threeftor)methyl(phenilsulphonilimino)-methylamino-25,26, 27,28-tetrapropoxy-calix[4]arene) on the activity of Ca2+,Mg2+-ATPase was investigated. The authors also examined the influence of calix[4]arene in different concentration on affinity of enzyme (Ca2+,Mg2+-ATPase) for the ATP and ions of Mg and Ca, and its influence on cooperative effect and maximum velocity of ATP hydrolysis. It is shown that calix[4]arene does not influence the affinity of Ca2+,Mg2+-ATPase for the ATP, which means that these two compounds have different binding centers. Also calix[4]arene has no influence on affinity and cooperative effect of Ca ions, if it is used in concentration lower than 50 µM. Calix[4]arene slightly increases coefficient of Ca2+,Mg2+-ATPase activation by magnesium chloride. In all three cases, where ATP, Mg and Ca ions are used to test the impact of calix[4]arene, maximum velocity of ATP hydrolysis significantly decreases. All these results clarify that calix[4]arene implements its inhibitory action through mechanism of uncompetitive inhibition of Ca2+,Mg2+-ATPase activity. |
Databáze: | OpenAIRE |
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