CircMED13L_012 promotes lung adenocarcinoma progression by upregulation of MAPK8 mediated by miR-433-3p
| Autor: | Wujin Li, Yangyun Huang, Guanying Zheng, Tianxing Guo, Lihuan Zhu, Xiaojie Pan, Wenshu Chen, Jianyuan Huang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
MAPK8 NSCLC lcsh:RC254-282 Metastasis Pathogenesis 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Genetics Medicine Stage (cooking) lcsh:QH573-671 neoplasms circMED13L_012 030304 developmental biology 0303 health sciences Reporter gene Lung business.industry lcsh:Cytology medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens respiratory tract diseases medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Cancer research Adenocarcinoma business Primary Research |
| Zdroj: | Cancer Cell International Cancer Cell International, Vol 21, Iss 1, Pp 1-12 (2021) |
| ISSN: | 1475-2867 |
| Popis: | Background Metastasis and disease refractoriness remain as major challenges for non-small cell lung cancer (NSCLC) treatment and understanding the underlying molecular mechanisms is of scientific and clinical value. Therefore, in this study, we aimed to explore the effects of circMED13L_012 on the proliferation, migration, invasion and drug-resistance of NSCLC tumor cells. Methods In this study, we utilized clinical samples and NSCLC cell lines to explore the association between circMED13L_012 expressions and tumor cell metastasis and chemo resistance. CCK8 and transwell assay were conducted to explore the impact of circMED13_012 on NSCLC tumor proliferation and migrative capabilities. Dual-luciferase reporter gene assay was conducted to validate the circMED13L_012 interaction network. Results Our results demonstrated that circMED13L_012 exhibited significantly elevated average level in our clinical samples of NSCLC, compared with normal tissues. circMED13L_012 level was positively correlated with disease stage and metastatic status. Increased circMED13L_012 expression was associated with the enhanced migration, proliferation and chemo resistance of NSCLC cell lines. Further experiments indicated that circMED13L_012 promoted malignant behavior of NSCLC tumor cells by targeting MAPK8 through modulation miR-433-3p expression. Conclusions Our study for the first time demonstrated that circMED13L_012–miR-433-3p–MAPK8 axis played important role for NSCLC pathogenesis, which could be potential therapeutic target for the development of future NSCLC treatment. |
| Databáze: | OpenAIRE |
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