Self assembled hyaluronic acid nanoparticles as a potential carrier for targeting the inflamed intestinal mucosa
| Autor: | Motahareh Esmaeili, Seyed Yaser Vafaei, Rassoul Dinarvand, Fatemeh Atyabi, Mohsen Amini, Seyed Naser Ostad |
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| Rok vydání: | 2016 |
| Předmět: |
Polymers and Plastics
Anti-Inflammatory Agents 02 engineering and technology Pharmacology 010402 general chemistry 01 natural sciences Flow cytometry Mice chemistry.chemical_compound Intestinal mucosa Hyaluronic acid Materials Chemistry medicine Animals Humans Hyaluronic Acid Intestinal Mucosa Budesonide Inflammation Drug Carriers biology medicine.diagnostic_test Chemistry Organic Chemistry CD44 Biological Transport 021001 nanoscience & nanotechnology 0104 chemical sciences Drug Liberation Hyaluronan Receptors Gene Expression Regulation Targeted drug delivery Cell culture NIH 3T3 Cells biology.protein Nanoparticles Caco-2 Cells Nanocarriers 0210 nano-technology Drug carrier Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Carbohydrate Polymers. 144:371-381 |
| ISSN: | 0144-8617 |
| Popis: | To develop a nanoparticulate drug carrier for targeting of the inflamed intestinal mucosa, amphiphilic hyaluronic acid (HA) conjugates were synthesized, which could form self-assembled nanoparticles (NPs) in aqueous solution and budesonide (BDS) was loaded into the HANPs. Their particle sizes were in the range of 177 to 293 nm with negative surface charge. The model of inflammatory CACO-2 cells was utilized to investigate the therapeutic potential of budesonide loaded HA nanocarriers. The highest expression of CD44 receptors was found on inflamed Caco-2 cells, as determined by flow cytometry. FITC-labeled HANPs revealed greater uptake in inflamed CACO-2 cells compared to untreated CACO-2 and CD44-negative cell lines, NIH3T3. BDS loaded HANPs displayed almost no toxicity indicating HANPs are excellent biocompatible nano-carriers. BDS loaded HANPs demonstrated higher anti-inflammatory effect on IL-8 and TNF-α secretion in inflamed cell model compared to the same dose of free drug. These results revealed the promising potential of HA nanoparticles as a targeted drug delivery system for IBD treatment. |
| Databáze: | OpenAIRE |
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