The mechanism of damage to the posterior silk gland by trace amounts of acetamiprid in the silkworm, Bombyx mori
| Autor: | Mengxue Li, Yilong Fang, Haina Sun, Bing Li, Tingting Mao, Minli Dai, Zhengting Lu, Jianwei Qu, Wentao Ye, Fanchi Li, Dandan Bian |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Health Toxicology and Mutagenesis media_common.quotation_subject Silk Fibroin Insect 01 natural sciences Acetamiprid 03 medical and health sciences chemistry.chemical_compound Neonicotinoids Bombyx mori Gene expression Animals Gene media_common biology Chemistry fungi General Medicine Metabolism biology.organism_classification Bombyx 010602 entomology 030104 developmental biology SILK Biochemistry Insect Proteins Fibroins Agronomy and Crop Science |
| Zdroj: | Pesticide biochemistry and physiology. 170 |
| ISSN: | 1095-9939 |
| Popis: | Acetamiprid is a new neonicotinoid insecticide widely used in the prevention and control of pests in agriculture. However, its residues in the environment affect the cocooning of the silkworm, Bombyx mori (B. mori), a non-target insect. To investigate the mechanism of damage, B. mori larvae were fed with trace amounts of acetamiprid (0.15 mg/L). At 96 h after exposure, the larvae showed signs of poisoning and decreased body weight, resulting in reduced survival and ratio of cocoon shell. At 48 h and 96 h after exposure, the residues in the posterior silk gland (PSG), which is responsible for synthesizing silk fibroin, were 0.72 μg/mg and 1.21 μg/mg, respectively, as measured by high performance liquid chromatography, indicating that acetamiprid can accumulate in the PSG. Moreover, pathological sections and transmission electron microscopy also demonstrate the damage of the PSG by acetamiprid. Digital gene expression (DGE) and KEGG pathway enrichment analysis revealed that genes related to metabolism, stress responses and inflammation were significantly up-regulated after exposure. Quantitative RT-PCR analysis showed that the transcript levels of FMBP-1 and FTZ-F1 (transcription factors for synthesizing silk protein) were up-regulated by 2.55-and 1.56-fold, respectively, and the transcript levels of fibroin heavy chain ( Fib-H), fibroin light chain (Fib-L), P25, Bmsage and Bmdimm were down-regulated by 0.75-, 0.76-, 0.65-, 0.44- and 0.40-fold, respectively. The results indicate that accumulated acetamiprid causes damage to the PSG and leads to reduced expression of genes responsible for synthesizing silk fibroin. Our data provide reference for evaluating the safety of acetamiprid residues in the environment for non-target insects. |
| Databáze: | OpenAIRE |
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