In Untreated HIV-1–Infected Children, PBMC-Associated HIV DNA Levels and Cell-Free HIV RNA Levels Are Correlated to Distinct T-lymphocyte Populations
| Autor: | Céline Didier, Christine Rouzioux, Stéphane Blanche, Marianne Burgard, Daniel Scott-Algara, Yves Rivière, Florence Buseyne |
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| Přispěvatelé: | Régulation des Infections Rétrovirales, Institut Pasteur [Paris], Infections à Vih, Réservoirs, Pharmacologie des Antirétroviraux et Prévention de la Transmission Mère Enfant, Université Paris Descartes - Paris 5 (UPD5), Laboratoire de Virologie [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service d'immuno-hématologie pédiatrique [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Immunopathologie Virale, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), This work was supported by Pasteur Institute, Agence Nationale de Recherches sur le SIDA et les hépatites virales, Sidaction, and the Paediatric AIDS Foundation., Assistance publique - Hôpitaux de Paris (AP-HP) - Hôpital Necker - Enfants Malades - Université Paris Descartes - Paris 5 (UPD5), Service d'immunologie, hématologie, Centre National de la Recherche Scientifique (CNRS) - Institut Pasteur [Paris], Université Paris Descartes - Paris 5 ( UPD5 ), Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), CHU Necker - Enfants Malades [AP-HP]-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Necker - Enfants Malades [AP-HP], Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2010 |
| Předmět: |
HIV Infections
MESH: Flow Cytometry CD8-Positive T-Lymphocytes MESH: CD4-Positive T-Lymphocytes/virology Polymerase Chain Reaction MESH: DNA Viral/immunology 0302 clinical medicine MESH : Child T-Lymphocyte Subsets [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases MESH: Child Immunopathology Pharmacology (medical) Child MESH : RNA Viral/immunology 0303 health sciences MESH: DNA Viral/blood MESH: RNA Viral/blood virus diseases MESH : Infant Flow Cytometry MESH: HIV Infections/virology MESH : Enzyme-Linked Immunosorbent Assay MESH: Infant 3. Good health MESH : CD4-Positive T-Lymphocytes/virology [ SDV.MHEP.MI ] Life Sciences [q-bio]/Human health and pathology/Infectious diseases MESH: CD8-Positive T-Lymphocytes/immunology [SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology Child Preschool [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Lentivirus HIV RNA MESH : HIV-1/immunology HIV-specific T lymphocytes Human leukocyte antigen [ SDV.MP.VIR ] Life Sciences [q-bio]/Microbiology and Parasitology/Virology Statistics Nonparametric MESH: CD4-Positive T-Lymphocytes/immunology 03 medical and health sciences MESH : Adolescent MESH : HIV Infections/virology Humans MESH : CD8-Positive T-Lymphocytes/virology MESH : DNA Viral/genetics [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology MESH : Leukocytes Mononuclear/virology Perinatal HIV infection MESH: Adolescent MESH: HIV-1/immunology MESH: Humans MESH : Humans MESH: Child Preschool Infant RNA MESH: Polymerase Chain Reaction MESH: HIV Infections/immunology medicine.disease Virology MESH: T-Lymphocyte Subsets/virology MESH: Leukocytes Mononuclear/immunology MESH : CD4-Positive T-Lymphocytes/immunology DNA Viral Immunology HIV-1 Leukocytes Mononuclear MESH : RNA Viral/genetics MESH: Female CD4-Positive T-Lymphocytes [ SDV.MHEP.PED ] Life Sciences [q-bio]/Human health and pathology/Pediatrics MESH : Leukocytes Mononuclear/immunology MESH : Child Preschool [ SDV.IMM.IA ] Life Sciences [q-bio]/Immunology/Adaptive immunology MESH : HIV Infections/immunology MESH: DNA Viral/genetics MESH : RNA Viral/blood MESH : Female 030212 general & internal medicine MESH : Polymerase Chain Reaction MESH : T-Lymphocyte Subsets/virology MESH: Statistics Nonparametric biology MESH : HIV-1/genetics MESH: Enzyme-Linked Immunosorbent Assay MESH: CD8-Positive T-Lymphocytes/virology Infectious Diseases MESH : DNA Viral/immunology MESH : Immunophenotyping RNA Viral Female MESH: Leukocytes Mononuclear/virology MESH: RNA Viral/genetics MESH : DNA Viral/blood Adolescent MESH: Immunophenotyping MESH : Flow Cytometry Enzyme-Linked Immunosorbent Assay Virus Immunophenotyping HIV DNA Acquired immunodeficiency syndrome (AIDS) MESH: T-Lymphocyte Subsets/immunology medicine immune activation MESH : CD8-Positive T-Lymphocytes/immunology MESH : Statistics Nonparametric MESH: RNA Viral/immunology 030304 developmental biology [SDV.MHEP.PED]Life Sciences [q-bio]/Human health and pathology/Pediatrics MESH: HIV-1/genetics T lymphocyte biology.organism_classification MESH : T-Lymphocyte Subsets/immunology CD8 |
| Zdroj: | Journal of Acquired Immune Deficiency Syndromes Journal of Acquired Immune Deficiency Syndromes, Lippincott, Williams & Wilkins, 2010, 53 (5), pp.553-563. ⟨10.1097/QAI.0b013e3181cf060f⟩ Journal of Acquired Immune Deficiency Syndromes, 2010, 53 (5), pp.553-63. <10.1097/QAI.0b013e3181cf060f> Journal of Acquired Immune Deficiency Syndromes, Lippincott, Williams & Wilkins, 2010, 53 (5), pp.553-563. 〈10.1097/QAI.0b013e3181cf060f〉 Journal of Acquired Immune Deficiency Syndromes-JAIDS Journal of Acquired Immune Deficiency Syndromes-JAIDS, 2010, 53 (5), pp.553-563. ⟨10.1097/QAI.0b013e3181cf060f⟩ |
| ISSN: | 1525-4135 1944-7884 |
| DOI: | 10.1097/qai.0b013e3181cf060f |
| Popis: | International audience; Background: Clinical studies support biologically independent roles of cell-free HIV particles and HIV-infected cells in disease progression. The associations between the level of infected cells and immune markers have been poorly studied, particularly in perinatally infected children. Objective: We tested the hypothesis that independent roles of cell-free virus and infected cells in HIV pathogenesis should be revealed by different associations between each of them and specific immune markers. Methods: Levels of HIV RNA and DNA, HIV-specific CD8 T lymphocytes, activated and naïve/memory T lymphocytes were determined in 44 untreated HIV-1-infected children. Pearson’s partial correlation coefficients were used to assess associations between the variables. Results: Here we provide new information, by showing a direct correlation between the percentages of CD4+HLA-DR+ lymphocytes and HIV DNA levels. Furthermore, higher HIV-specific CD8 T lymphocyte frequencies were associated with lower HIV DNA levels. In contrast, CD8+38+ lymphocytes and memory CD4 lymphocytes were correlated only to the HIV RNA level. All correlations were independent of age and CD4 depletion.Conclusion: Several immune markers were correlated to either the HIV RNA or the HIV DNA level, but never to both of them, supporting the concept that cell-free virus and infected cells play different roles in HIV-1 immunopathogenesis. |
| Databáze: | OpenAIRE |
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