Mycobacterium tuberculosis retains viability in RNAlater buffer but not in GTC-TCEP and DNA/RNA Shield

Autor:	L. Krausser, S.M. Braet, Z. Benaamar, M. Van Dyck-Lippens, B.C. de Jong, L. Rigouts
Rok vydání:	2023
Předmět:	Microbiology (medical) Infectious Diseases Human medicine General Medicine Biology
Zdroj:	Diagnostic microbiology and infectious disease
ISSN:	0732-8893
DOI:	10.1016/j.diagmicrobio.2023.115905
Popis:	Efficient inactivation of clinical samples containing mycobacteria is crucial for biosafety during shipment and handling. When stored in RNAlater, Mycobacterium tuberculosis H37Ra remains viable, and our results sug-gest that at-20 degrees C and 4 degrees C changes in the mycobacterial transcriptome are possible. Only GTC-TCEP and DNA/RNA Shield inactivate sufficiently for shipment.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ac34fc6bb1238f016acfa28567e4820 https://doi.org/10.1016/j.diagmicrobio.2023.115905 Zobrazit plný text záznamu Full Text from ScienceDirect