Cryptococcus neoformans VNII as the Main Cause of Cryptococcosis in Domestic Cats from Rio de Janeiro, Brazil
| Autor: | Isabella Dib Ferreira Gremião, Fausto Klabund Ferraris, Isabela Maria da Silva Antonio, Carlos Roberto Sobrinho do Nascimento, Sandro Antonio Pereira, Isabel Cristina Fábregas Bonna, Fábio Brito-Santos, Rosani Santos Reis, Luciana Trilles |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Cryptococcus neoformans Species complex CATS cryptococcosis biology Molecular epidemiology QH301-705.5 Cryptococcus Plant Science biology.organism_classification medicine.disease molecular epidemiology Article Microbiology domestic cats Cryptococcosis Genotype medicine Multilocus sequence typing Biology (General) Ecology Evolution Behavior and Systematics MLST |
| Zdroj: | Journal of Fungi, Vol 7, Iss 980, p 980 (2021) Journal of Fungi Volume 7 Issue 11 |
| ISSN: | 2309-608X |
| DOI: | 10.3390/jof7110980 |
| Popis: | Cryptococcosis is a systemic fungal disease acquired from contaminated environments with propagules of the basidiomycetous yeasts of the Cryptococcus neoformans and C. gattii species complexes. The C. neoformans species complex classically comprises four major molecular types (VNI, VNII, VNIII, and VNIV), and the C. gattii species complex comprises another four (VGI, VGII, VGIII, and VGIV) and the newly identified molecular type VGV. These major molecular types differ in their epidemiological and ecological features, clinical presentations, and therapeutic outcomes. Generally, the most common isolated types are VNI, VGI, and VGII. The epidemiological profile of cryptococcosis in domestic cats is poorly studied and cats can be the sentinels for human infections. Therefore, the present study aimed to determine the molecular characterization of Cryptococcus spp. isolated from domestic cats and their dwellings in the metropolitan area of Rio de Janeiro, Brazil. A total of 36 Cryptococcus spp. strains, both clinical and environmental, from 19 cats were subtyped using multilocus sequence typing (MLST). The ploidy was identified using flow cytometry and the mating type was determined through amplification with specific pheromone primers. All strains were mating type alpha and 6/36 were diploid (all VNII). Most isolates (63.88%) were identified as VNII, a rare molecular type, leading to the consideration that this genotype is more likely related to skin lesions, since there was a high percentage (68.75%) of cats with skin lesions, which is also considered rare. Further studies regarding the molecular epidemiology of cryptococcosis in felines are still needed to clarify the reason for the large proportion of the rare molecular type VNII causing infections in cats. |
| Databáze: | OpenAIRE |
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