Automated high-throughput dense matrix protein folding screen using a liquid handling robot combined with microfluidic capillary electrophoresis
| Autor: | Kenneth W. Walker, Philip An, Dwight Winters |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Protein Folding Microfluidics Computational biology law.invention 03 medical and health sciences Capillary electrophoresis law Escherichia coli Native state 030102 biochemistry & molecular biology biology Chemistry Electrophoresis Capillary Robotics Folding (DSP implementation) Recombinant Proteins High-Throughput Screening Assays 030104 developmental biology Solubility Biochemistry Liquid handling robot Recombinant DNA biology.protein Protein folding Protein A Biotechnology |
| Zdroj: | Protein Expression and Purification. 120:138-147 |
| ISSN: | 1046-5928 |
| DOI: | 10.1016/j.pep.2015.11.015 |
| Popis: | Modern molecular genetics technology has made it possible to swiftly sequence, clone and mass-produce recombinant DNA for the purpose of expressing heterologous genes of interest; however, recombinant protein production systems have struggled to keep pace. Mammalian expression systems are typically favored for their ability to produce and secrete proteins in their native state, but bacterial systems benefit from rapid cell line development and robust growth. The primary drawback to prokaryotic expression systems are that recombinant proteins are generally not secreted at high levels or correctly folded, and are often insoluble, necessitating post-expression protein folding to obtain the active product. In order to harness the advantages of prokaryotic expression, high-throughput methods for executing protein folding screens and the subsequent analytics to identify lead conditions are required. Both of these tasks can be accomplished using a Biomek 3000 liquid handling robot to prepare the folding screen and to subsequently prepare the reactions for assessment using Caliper microfluidic capillary electrophoresis. By augmenting a protein folding screen with automation, the primary disadvantage of Escherichia coli expression has been mitigated, namely the labor intensive identification of the required protein folding conditions. Furthermore, a rigorous, quantitative method for identifying optimal protein folding buffer aids in the rapid development of an optimal production process. |
| Databáze: | OpenAIRE |
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