Molecular characterization of Brucella species from Zimbabwe
| Autor: | Ayesha Hassim, Maphuti Betty Ledwaba, Kgaugelo Edward Lekota, Henriette van Heerden, Calvin Gomo, Gilles Vergnaud, Philippe Le Flèche |
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| Přispěvatelé: | Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Séquence, Structure et Fonction des ARN (SSFA), Département Biologie des Génomes (DBG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Bacterial Diseases
0301 basic medicine Swine Biovar [SDV]Life Sciences [q-bio] RC955-962 Brucella abortus Artificial Gene Amplification and Extension Minisatellite Repeats Pathology and Laboratory Medicine Polymerase Chain Reaction Genome law.invention Geographical Locations 0302 clinical medicine law Zoonoses Arctic medicine. Tropical medicine Medicine and Health Sciences Phylogeny Polymerase chain reaction Data Management Mammals Swine Diseases Genetics biology Eukaryota Agriculture Phylogenetic Analysis Ruminants Bacterial Pathogens Phylogenetics Variable number tandem repeat Infectious Diseases Medical Microbiology Vertebrates Pathogens Public aspects of medicine RA1-1270 Research Article Neglected Tropical Diseases Zimbabwe Computer and Information Sciences Livestock Genotype 030231 tropical medicine Cattle Diseases Sheep Diseases Genomics Brucella Multiple Loci VNTR Analysis Research and Analysis Methods Microbiology Brucellosis 03 medical and health sciences Bovines Brucella melitensis Animals Evolutionary Systematics Molecular Biology Techniques Microbial Pathogens Molecular Biology Taxonomy Whole genome sequencing Evolutionary Biology Sheep Bacteria Organisms Public Health Environmental and Occupational Health Biology and Life Sciences Tropical Diseases biology.organism_classification 030104 developmental biology Amniotes People and Places Africa Cattle Genome Bacterial |
| Zdroj: | PLoS Neglected Tropical Diseases, Vol 13, Iss 5, p e0007311 (2019) PLoS Neglected Tropical Diseases PLoS Neglected Tropical Diseases, 2019, 13 (5), pp.e0007311. ⟨10.1371/journal.pntd.0007311⟩ PLoS Neglected Tropical Diseases, Public Library of Science, 2019, 13 (5), pp.e0007311. ⟨10.1371/journal.pntd.0007311⟩ |
| ISSN: | 1935-2735 1935-2727 |
| DOI: | 10.1371/journal.pntd.0007311⟩ |
| Popis: | Brucella abortus and B. melitensis have been reported in several studies in animals in Zimbabwe but the extent of the disease remains poorly known. Thus, characterizing the circulating strains is a critical first step in understanding brucellosis in the country. In this study we used an array of molecular assays including AMOS-PCR, Bruce-ladder, multiple locus variable number tandem repeats analysis (MLVA) and single nucleotide polymorphisms from whole genome sequencing (WGS-SNP) to characterize Brucella isolates to the species, biovar, and individual strain level. Sixteen Brucella strains isolated in Zimbabwe at the Central Veterinary laboratory from various hosts were characterized using all or some of these assays. The strains were identified as B. ovis, B. abortus, B. canis and B. suis, with B. canis being the first report of this species in Zimbabwe. Zimbabwean strains identified as B. suis and B. abortus were further characterized with whole genome sequencing and were closely related to reference strains 1330 and 86/8/59, respectively. We demonstrate the range of different tests that can be performed from simple assays that can be run in laboratories lacking sophisticated instrumentation to whole genome analyses that currently require substantial expertise and infrastructure often not available in the developing world. Author summary Brucellosis is endemic in Zimbabwe. This article describes the use of various assays such as AMOS, Bruce-ladder, MLVA, and whole genome sequencing to characterize Brucella species isolated from different animals in Zimbabwe. Choice of which assays to use in the laboratory is generally done considering reproducibility, robustness, expertise and affordability in a given setting. As evidenced in this study, most laboratories in Africa lack resources especially finances, equipments and expertise to perform necessary tests for diagnosis and identification of specific pathogens. The study shows that the differentiation of species can be correctly concluded from the analysis with AMOS, Bruce-ladder and MLVA16 assays. Furthermore, MLVA16 can be used as an epidemiological tool and traceback of outbreaks. These PCR assays can therefore add to the control and eradication of brucellosis, since the Brucella species (B. ovis, B. abortus, B. suis and B. canis) existing in Zimbabwe could be identified and characterized. |
| Databáze: | OpenAIRE |
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