Localization of three forms of gonadotropin-releasing hormone in the brain and pituitary of the self-fertilizing fish, Kryptolebias marmoratus
Autor: | Yoshitaka Sakakura, Ayae Kuriu, Junpei Yamashita, Naoyuki Yamamoto, Ayano Yasuta, Masafumi Amano, Kataaki Okubo, Noriko Amiya |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
endocrine system
Physiology Gonadotropin-releasing hormone In situ hybridization Aquatic Science Biochemistry Gonadotropin-Releasing Hormone 03 medical and health sciences Animals Humans Hermaphroditic Organisms Killifish Amino Acid Sequence Self-fertilizing fish Peptide sequence Phylogeny 030304 developmental biology 0303 health sciences biology Base Sequence Killifishes Reproduction Brain 04 agricultural and veterinary sciences General Medicine biology.organism_classification Immunohistochemistry Cell biology Pituitary Gland GnRH GTH 040102 fisheries 0401 agriculture forestry and fisheries Midbrain tegmentum Luteinizing hormone Hormone |
Zdroj: | Fish Physiology and Biochemistry. 45(2):753-771 |
ISSN: | 0920-1742 |
Popis: | The localization of gonadotropin-releasing hormone (GnRH) in the brain and pituitary of the self-fertilizing mangrove killifish Kryptolebias marmoratus was examined by immunohistochemistry and in situ hybridization to understand its neuroendocrine system. The genome assembly of K. marmoratus did not have any sequence encoding GnRH1, but sequences encoding GnRH2 (chicken GnRH-II) and GnRH3 (salmon GnRH) were found. Therefore, GnRH1 was identified by in silico cloning. The deduced amino acid sequence of the K. marmoratus GnRH1 (mature peptide) was identical to that of the medaka GnRH. GnRH1 neurons were detected in the ventral part of the preoptic nucleus by immunohistochemistry and in situ hybridization, and GnRH1-immunoreactive (ir) fibers were observed throughout the brain. GnRH1-ir fibers were in close contact with luteinizing hormone (LH)-ir cells in the pituitary using double immunohistochemistry. GnRH2 neurons were detected in the midbrain tegmentum by immunohistochemistry and in situ hybridization. Although GnRH2-ir fibers were observed throughout the brain, they were not detected in the pituitary. GnRH3 neurons were detected in the lateral part of the ventral telencephalic area by both methods. GnRH3-ir fibers were observed throughout the brain, and a few GnRH3-ir fibers were in close contact with LH-ir cells in the pituitary. These results indicate that GnRH1 and possibly GnRH3 are responsible for gonadal maturation through LH secretion and that all three forms of GnRH function as neurotransmitters or neuromodulators in the brain of K. marmoratus. Fish Physiology and Biochemistry, 45(2), pp.753-771; 2019 |
Databáze: | OpenAIRE |
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