Systematic Discovery of Endogenous Human Ribonucleoprotein Complexes
| Autor: | Kevin Drew, Edward M. Marcotte, Anna L. Mallam, Jeffrey M. Schaub, John B. Wallingford, Yu Jin Jang, Ilya J. Finkelstein, Wisath Sae-Lee, Jonghwan Kim, Anna Battenhouse, Fan Tu |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Proteome Endogeny RNA-binding protein Computational biology Cell Fractionation Proteomics Interactome Article General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences Replication factor C 0302 clinical medicine Human disease Animals Humans Replication Protein C lcsh:QH301-705.5 030304 developmental biology Ribonucleoprotein 0303 health sciences Chemistry Reproducibility of Results RNA Centralspindlin complex Embryonic stem cell 3. Good health HEK293 Cells 030104 developmental biology Ribonucleoproteins lcsh:Biology (General) Multiprotein Complexes Nucleic Acid Conformation Function (biology) 030217 neurology & neurosurgery |
| Zdroj: | Cell Reports, Vol 29, Iss 5, Pp 1351-1368.e5 (2019) Cell reports |
| ISSN: | 2211-1247 |
| Popis: | SUMMARY RNA-binding proteins (RBPs) play essential roles in biology and are frequently associated with human disease. Although recent studies have systematically identified individual RNA-binding proteins, their higher-order assembly into ribonucleoprotein (RNP) complexes has not been systematically investigated. Here, we describe a proteomics method for systematic identification of RNP complexes in human cells. We identify 1,428 protein complexes that associate with RNA, indicating that more than 20% of known human protein complexes contain RNA. To explore the role of RNA in the assembly of each complex, we identify complexes that dissociate, change composition, or form stable protein-only complexes in the absence of RNA. We use our method to systematically identify cell-type-specific RNA-associated proteins in mouse embryonic stem cells and finally, distribute our resource, rna.MAP, in an easy-to-use online interface (rna.proteincomplexes.org). Our system thus provides a methodology for explorations across human tissues, disease states, and throughout all domains of life. In Brief Ribonucleoprotein (RNP) complexes carry out many essential biological processes. Mallam et al. developed differential fractionation (DIF-FRAC), a proteomics method to systematically discover RNP complexes. Using their method, they discovered previously unknown RNP complexes, classified complexes by their RNA-dependent stability, and identified previously unknown roles for RNA in known protein complexes. Graphical Abstract |
| Databáze: | OpenAIRE |
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