| Přispěvatelé: |
Gennaro, M. C., Abrigo, C., Angelino, S., Albert, U., Bogetto, F., Maina, G., Prolo, P., Ravizza, L. |
| Popis: |
The paper reports a sensitive Ion-Interaction Reverse Phase HPLC-UV method for the separation and determination of fluoxetine ((±)N-methyl-(4-(trifluoromethyl)phenoxy) benzene-propanamine) in human plasma. An ODS silica-based column is used as the stationary phase and the mobile phase consists of 5.00 mM octylamine in water/acetonitrile (60/40 v/v), at pH = 6.4, with UV detection at 230 nm. The method is selective towards potentially interferent drugs, such as paroxetine, amylsulpride, fluvoxamine, mianserin, imipramine, amitryptiline, cromipramine, maprotiline, haloperidol, flunitrazepam and diazepam. The proposed method was successfully applied to the determination of fluoxetine and norfluoxetine in plasma of some patients being treated for obsessive-compulsive disorders. |
